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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Akkermansia muciniphila 30S ribosomal protein S20 (rpsT) CSB-YP454958AZF
CSB-EP454958AZF
CSB-BP454958AZF
CSB-MP454958AZF
CSB-EP454958AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L16 (rplP) CSB-YP454959AZF
CSB-EP454959AZF
CSB-BP454959AZF
CSB-MP454959AZF
CSB-EP454959AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L2 (rplB) CSB-YP454960AZF
CSB-EP454960AZF
CSB-BP454960AZF
CSB-MP454960AZF
CSB-EP454960AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Sec-independent protein translocase protein TatC (tatC), partial CSB-YP454961AZF1
CSB-EP454961AZF1
CSB-BP454961AZF1
CSB-MP454961AZF1
CSB-EP454961AZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP454962AZF
CSB-EP454962AZF
CSB-BP454962AZF
CSB-MP454962AZF
CSB-EP454962AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila Chorismate synthase (aroC) CSB-YP454963AZF
CSB-EP454963AZF
CSB-BP454963AZF
CSB-MP454963AZF
CSB-EP454963AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 30S ribosomal protein S14 (rpsN) CSB-YP454964AZF
CSB-EP454964AZF
CSB-BP454964AZF
CSB-MP454964AZF
CSB-EP454964AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP454965AZF
CSB-EP454965AZF
CSB-BP454965AZF
CSB-MP454965AZF
CSB-EP454965AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L15 (rplO) CSB-YP454966AZF
CSB-EP454966AZF
CSB-BP454966AZF
CSB-MP454966AZF
CSB-EP454966AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila S-adenosylmethionine synthase (metK) CSB-YP454967AZF
CSB-EP454967AZF
CSB-BP454967AZF
CSB-MP454967AZF
CSB-EP454967AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akkermansia muciniphila 50S ribosomal protein L13 (rplM) CSB-YP454968AZF
CSB-EP454968AZF
CSB-BP454968AZF
CSB-MP454968AZF
CSB-EP454968AZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori S-ribosylhomocysteine lyase (luxS) CSB-YP454969HUZ
CSB-EP454969HUZ
CSB-BP454969HUZ
CSB-MP454969HUZ
CSB-EP454969HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Enolase (eno) CSB-YP454970HUZ
CSB-EP454970HUZ
CSB-BP454970HUZ
CSB-MP454970HUZ
CSB-EP454970HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Lysine--tRNA ligase (lysS), partial CSB-YP454971HUZ
CSB-EP454971HUZ
CSB-BP454971HUZ
CSB-MP454971HUZ
CSB-EP454971HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori S-adenosylmethionine synthase (metK) CSB-YP454972HUZ
CSB-EP454972HUZ
CSB-BP454972HUZ
CSB-MP454972HUZ
CSB-EP454972HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP454973HUZ
CSB-EP454973HUZ
CSB-BP454973HUZ
CSB-MP454973HUZ
CSB-EP454973HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP454974HUZ
CSB-EP454974HUZ
CSB-BP454974HUZ
CSB-MP454974HUZ
CSB-EP454974HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 50S ribosomal protein L27 (rpmA) CSB-YP454975HUZ
CSB-EP454975HUZ
CSB-BP454975HUZ
CSB-MP454975HUZ
CSB-EP454975HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori NH (3)-dependent NAD (+) synthetase CSB-YP454976HUZ
CSB-EP454976HUZ
CSB-BP454976HUZ
CSB-MP454976HUZ
CSB-EP454976HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosomal protein L11 methyltransferase (prmA) CSB-YP454977HUZ
CSB-EP454977HUZ
CSB-BP454977HUZ
CSB-MP454977HUZ
CSB-EP454977HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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