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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas oryzae pv. oryzae 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP454758XBC
CSB-EP454758XBC
CSB-BP454758XBC
CSB-MP454758XBC
CSB-EP454758XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae UPF0102 protein PXO_04380 (PXO_04380) CSB-YP454759XBC
CSB-EP454759XBC
CSB-BP454759XBC
CSB-MP454759XBC
CSB-EP454759XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Protein translocase subunit SecA (secA), partial CSB-YP454760XBC
CSB-EP454760XBC
CSB-BP454760XBC
CSB-MP454760XBC
CSB-EP454760XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP454761XBC
CSB-EP454761XBC
CSB-BP454761XBC
CSB-MP454761XBC
CSB-EP454761XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Glutamyl-tRNA reductase (hemA) CSB-YP454762XBC
CSB-EP454762XBC
CSB-BP454762XBC
CSB-MP454762XBC
CSB-EP454762XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 30S ribosomal protein S7 (rpsG) CSB-YP454763XBC
CSB-EP454763XBC
CSB-BP454763XBC
CSB-MP454763XBC
CSB-EP454763XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 50S ribosomal protein L14 (rplN) CSB-YP454764XBC
CSB-EP454764XBC
CSB-BP454764XBC
CSB-MP454764XBC
CSB-EP454764XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP454765XBC
CSB-EP454765XBC
CSB-BP454765XBC
CSB-MP454765XBC
CSB-EP454765XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP454766XBC
CSB-EP454766XBC
CSB-BP454766XBC
CSB-MP454766XBC
CSB-EP454766XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Arginine--tRNA ligase (argS), partial CSB-YP454767XBC
CSB-EP454767XBC
CSB-BP454767XBC
CSB-MP454767XBC
CSB-EP454767XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 7-cyano-7-deazaguanine synthase (queC) CSB-YP454768XBC
CSB-EP454768XBC
CSB-BP454768XBC
CSB-MP454768XBC
CSB-EP454768XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Aspartate--tRNA ligase (aspS), partial CSB-YP454769XBC
CSB-EP454769XBC
CSB-BP454769XBC
CSB-MP454769XBC
CSB-EP454769XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP454770XBC
CSB-EP454770XBC
CSB-BP454770XBC
CSB-MP454770XBC
CSB-EP454770XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP454771XBC
CSB-EP454771XBC
CSB-BP454771XBC
CSB-MP454771XBC
CSB-EP454771XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Alanine--tRNA ligase (alaS), partial CSB-YP454772XBC
CSB-EP454772XBC
CSB-BP454772XBC
CSB-MP454772XBC
CSB-EP454772XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae D-galactonate dehydratase (dgoD) CSB-YP454773XBC
CSB-EP454773XBC
CSB-BP454773XBC
CSB-MP454773XBC
CSB-EP454773XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Ribosome maturation factor RimP (rimP) CSB-YP454774XBC
CSB-EP454774XBC
CSB-BP454774XBC
CSB-MP454774XBC
CSB-EP454774XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP454775XBC
CSB-EP454775XBC
CSB-BP454775XBC
CSB-MP454775XBC
CSB-EP454775XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Tryptophan synthase beta chain (trpB) CSB-YP454776XBC
CSB-EP454776XBC
CSB-BP454776XBC
CSB-MP454776XBC
CSB-EP454776XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae Deoxycytidine triphosphate deaminase (dcd) CSB-YP454777XBC
CSB-EP454777XBC
CSB-BP454777XBC
CSB-MP454777XBC
CSB-EP454777XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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