Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Phytoplasma mali 50S ribosomal protein L34 (rpmH) CSB-YP455559EVH
CSB-EP455559EVH
CSB-BP455559EVH
CSB-MP455559EVH
CSB-EP455559EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 30S ribosomal protein S7 (rpsG) CSB-YP455560EVH
CSB-EP455560EVH
CSB-BP455560EVH
CSB-MP455560EVH
CSB-EP455560EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L21 (rplU) CSB-YP455561EVH
CSB-EP455561EVH
CSB-BP455561EVH
CSB-MP455561EVH
CSB-EP455561EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali 50S ribosomal protein L14 (rplN) CSB-YP455562EVH
CSB-EP455562EVH
CSB-BP455562EVH
CSB-MP455562EVH
CSB-EP455562EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Asparagine--tRNA ligase (asnS) CSB-YP455563EVH
CSB-EP455563EVH
CSB-BP455563EVH
CSB-MP455563EVH
CSB-EP455563EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytoplasma mali Chromosomal replication initiator protein DnaA (dnaA) CSB-YP455564EVH
CSB-EP455564EVH
CSB-BP455564EVH
CSB-MP455564EVH
CSB-EP455564EVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP455565DZS
CSB-EP455565DZS
CSB-BP455565DZS
CSB-MP455565DZS
CSB-EP455565DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis GTPase Der (der) CSB-YP455566DZS
CSB-EP455566DZS
CSB-BP455566DZS
CSB-MP455566DZS
CSB-EP455566DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis 50S ribosomal protein L31 type B (rpmE2) CSB-YP455567DZS
CSB-EP455567DZS
CSB-BP455567DZS
CSB-MP455567DZS
CSB-EP455567DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis DNA ligase (ligA), partial CSB-YP455568DZS
CSB-EP455568DZS
CSB-BP455568DZS
CSB-MP455568DZS
CSB-EP455568DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis LexA repressor (lexA) CSB-YP455569DZS
CSB-EP455569DZS
CSB-BP455569DZS
CSB-MP455569DZS
CSB-EP455569DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Serine--tRNA ligase (serS) CSB-YP455570DZS
CSB-EP455570DZS
CSB-BP455570DZS
CSB-MP455570DZS
CSB-EP455570DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Adenine deaminase (RALTA_A0998) CSB-YP455571DZS
CSB-EP455571DZS
CSB-BP455571DZS
CSB-MP455571DZS
CSB-EP455571DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP455572DZS
CSB-EP455572DZS
CSB-BP455572DZS
CSB-MP455572DZS
CSB-EP455572DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Urease subunit beta (ureB) CSB-YP455573DZS
CSB-EP455573DZS
CSB-BP455573DZS
CSB-MP455573DZS
CSB-EP455573DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Bifunctional protein FolD (folD) CSB-YP455574DZS
CSB-EP455574DZS
CSB-BP455574DZS
CSB-MP455574DZS
CSB-EP455574DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP455575DZS
CSB-EP455575DZS
CSB-BP455575DZS
CSB-MP455575DZS
CSB-EP455575DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Deoxycytidine triphosphate deaminase (dcd) CSB-YP455576DZS
CSB-EP455576DZS
CSB-BP455576DZS
CSB-MP455576DZS
CSB-EP455576DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis UPF0234 protein RALTA_A2535 (RALTA_A2535) CSB-YP455577DZS
CSB-EP455577DZS
CSB-BP455577DZS
CSB-MP455577DZS
CSB-EP455577DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP455578DZS
CSB-EP455578DZS
CSB-BP455578DZS
CSB-MP455578DZS
CSB-EP455578DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>