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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Assembly factor CBP4 (CBP4), partial CSB-YP455359SVP
CSB-EP455359SVP
CSB-BP455359SVP
CSB-MP455359SVP
CSB-EP455359SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 39, mitochondrial (AIM39), partial CSB-YP455360SVP
CSB-EP455360SVP
CSB-BP455360SVP
CSB-MP455360SVP
CSB-EP455360SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Sphingoid long-chain base transporter RSB1 (RSB1), partial CSB-YP455361SVP1
CSB-EP455361SVP1
CSB-BP455361SVP1
CSB-MP455361SVP1
CSB-EP455361SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UPF0507 protein SCRG_01893 (SCRG_01893), partial CSB-YP455362SVP
CSB-EP455362SVP
CSB-BP455362SVP
CSB-MP455362SVP
CSB-EP455362SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Defect at low temperature protein 1 (DLT1), partial CSB-YP455363SVP
CSB-EP455363SVP
CSB-BP455363SVP
CSB-MP455363SVP
CSB-EP455363SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 36, mitochondrial (AIM36), partial CSB-YP455364SVP1
CSB-EP455364SVP1
CSB-BP455364SVP1
CSB-MP455364SVP1
CSB-EP455364SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 4 (AIM4) CSB-YP455365SVP
CSB-EP455365SVP
CSB-BP455365SVP
CSB-MP455365SVP
CSB-EP455365SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Protein SUS1 (SUS1) CSB-YP455366SVP
CSB-EP455366SVP
CSB-BP455366SVP
CSB-MP455366SVP
CSB-EP455366SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting/targeting protein PEP1 (PEP1), partial CSB-YP455367SVP
CSB-EP455367SVP
CSB-BP455367SVP
CSB-MP455367SVP
CSB-EP455367SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 37, mitochondrial (AIM37), partial CSB-YP455368SVP1
CSB-EP455368SVP1
CSB-BP455368SVP1
CSB-MP455368SVP1
CSB-EP455368SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Required for respiratory growth protein 9, mitochondrial (RRG9) CSB-YP455369SVP
CSB-EP455369SVP
CSB-BP455369SVP
CSB-MP455369SVP
CSB-EP455369SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosome-releasing factor 2, mitochondrial (MEF2), partial CSB-YP455370SVP
CSB-EP455370SVP
CSB-BP455370SVP
CSB-MP455370SVP
CSB-EP455370SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Nucleolar protein 9 (NOP9), partial CSB-YP455371SVP
CSB-EP455371SVP
CSB-BP455371SVP
CSB-MP455371SVP
CSB-EP455371SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Biogenesis of lysosome-related organelles complex 1 subunit BLI1 (BLI1) CSB-YP455372SVP
CSB-EP455372SVP
CSB-BP455372SVP
CSB-MP455372SVP
CSB-EP455372SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Transcriptional activator FLO8 (FLO8), partial CSB-YP455373SVP
CSB-EP455373SVP
CSB-BP455373SVP
CSB-MP455373SVP
CSB-EP455373SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Altered inheritance of mitochondria protein 18, mitochondrial (AIM18) CSB-YP455374SVP
CSB-EP455374SVP
CSB-BP455374SVP
CSB-MP455374SVP
CSB-EP455374SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative uncharacterized protein SCRG_04940 (SCRG_04940), partial CSB-YP455375SVP1
CSB-EP455375SVP1
CSB-BP455375SVP1
CSB-MP455375SVP1
CSB-EP455375SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-related modifier 1 (URM1) CSB-YP455376SVP
CSB-EP455376SVP
CSB-BP455376SVP
CSB-MP455376SVP
CSB-EP455376SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Autophagy-related protein 32 (ATG32), partial CSB-YP455377SVP1
CSB-EP455377SVP1
CSB-BP455377SVP1
CSB-MP455377SVP1
CSB-EP455377SVP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Regulator of rDNA transcription protein 5 (RRT5) CSB-YP455378SVP
CSB-EP455378SVP
CSB-BP455378SVP
CSB-MP455378SVP
CSB-EP455378SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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