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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shigella boydii serotype 18 DNA replication and repair protein recF (recF) CSB-YP454858SYZ
CSB-EP454858SYZ
CSB-BP454858SYZ
CSB-MP454858SYZ
CSB-EP454858SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0352 protein yejL (yejL) CSB-YP454859SYZ
CSB-EP454859SYZ
CSB-BP454859SYZ
CSB-MP454859SYZ
CSB-EP454859SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 50S ribosomal protein L25 (rplY) CSB-YP454860SYZ
CSB-EP454860SYZ
CSB-BP454860SYZ
CSB-MP454860SYZ
CSB-EP454860SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Biotin synthase (bioB) CSB-YP454861SYZ
CSB-EP454861SYZ
CSB-BP454861SYZ
CSB-MP454861SYZ
CSB-EP454861SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Der GTPase-activating protein YihI (yihI) CSB-YP454862SYZ
CSB-EP454862SYZ
CSB-BP454862SYZ
CSB-MP454862SYZ
CSB-EP454862SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 HTH-type transcriptional activator rhaS (rhaS) CSB-YP454863SYZ
CSB-EP454863SYZ
CSB-BP454863SYZ
CSB-MP454863SYZ
CSB-EP454863SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative bifunctional polymyxin resistance protein ArnA (arnA), partial CSB-YP454864SYZ
CSB-EP454864SYZ
CSB-BP454864SYZ
CSB-MP454864SYZ
CSB-EP454864SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP454865SYZ1
CSB-EP454865SYZ1
CSB-BP454865SYZ1
CSB-MP454865SYZ1
CSB-EP454865SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Penicillin-insensitive murein endopeptidase (mepA) CSB-YP454866SYZ
CSB-EP454866SYZ
CSB-BP454866SYZ
CSB-MP454866SYZ
CSB-EP454866SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Met repressor (metJ) CSB-YP454867SYZ
CSB-EP454867SYZ
CSB-BP454867SYZ
CSB-MP454867SYZ
CSB-EP454867SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Acetylglutamate kinase (argB) CSB-YP454868SYZ
CSB-EP454868SYZ
CSB-BP454868SYZ
CSB-MP454868SYZ
CSB-EP454868SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP454869SYZ
CSB-EP454869SYZ
CSB-BP454869SYZ
CSB-MP454869SYZ
CSB-EP454869SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP454870SYZ
CSB-EP454870SYZ
CSB-BP454870SYZ
CSB-MP454870SYZ
CSB-EP454870SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Pyridoxine kinase (pdxK) CSB-YP454871SYZ
CSB-EP454871SYZ
CSB-BP454871SYZ
CSB-MP454871SYZ
CSB-EP454871SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Glycerol-3-phosphate acyltransferase (plsB), partial CSB-YP454872SYZ
CSB-EP454872SYZ
CSB-BP454872SYZ
CSB-MP454872SYZ
CSB-EP454872SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative mannosyl-3-phosphoglycerate phosphatase (SbBS512_E1078) CSB-YP454873SYZ
CSB-EP454873SYZ
CSB-BP454873SYZ
CSB-MP454873SYZ
CSB-EP454873SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP454874SYZ
CSB-EP454874SYZ
CSB-BP454874SYZ
CSB-MP454874SYZ
CSB-EP454874SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Cytoskeleton protein rodZ (rodZ), partial CSB-YP454875SYZ
CSB-EP454875SYZ
CSB-BP454875SYZ
CSB-MP454875SYZ
CSB-EP454875SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Peptidase B (pepB) CSB-YP454876SYZ
CSB-EP454876SYZ
CSB-BP454876SYZ
CSB-MP454876SYZ
CSB-EP454876SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP454877SYZ
CSB-EP454877SYZ
CSB-BP454877SYZ
CSB-MP454877SYZ
CSB-EP454877SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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