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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella boydii serotype 18 L-ribulose-5-phosphate 4-epimerase UlaF (ulaF) CSB-YP454878SYZ
CSB-EP454878SYZ
CSB-BP454878SYZ
CSB-MP454878SYZ
CSB-EP454878SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Multidrug resistance protein MdtB (mdtB), partial CSB-YP454879SYZ
CSB-EP454879SYZ
CSB-BP454879SYZ
CSB-MP454879SYZ
CSB-EP454879SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Elongation factor 4 (lepA), partial CSB-YP454880SYZ
CSB-EP454880SYZ
CSB-BP454880SYZ
CSB-MP454880SYZ
CSB-EP454880SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein grpE (grpE) CSB-YP454881SYZ
CSB-EP454881SYZ
CSB-BP454881SYZ
CSB-MP454881SYZ
CSB-EP454881SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 RNA pyrophosphohydrolase (rppH) CSB-YP454882SYZ
CSB-EP454882SYZ
CSB-BP454882SYZ
CSB-MP454882SYZ
CSB-EP454882SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0307 protein YjgA (yjgA) CSB-YP454883SYZ
CSB-EP454883SYZ
CSB-BP454883SYZ
CSB-MP454883SYZ
CSB-EP454883SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0260 protein ycgN (ycgN) CSB-YP454884SYZ
CSB-EP454884SYZ
CSB-BP454884SYZ
CSB-MP454884SYZ
CSB-EP454884SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 L-fucose mutarotase (fucU) CSB-YP454885SYZ
CSB-EP454885SYZ
CSB-BP454885SYZ
CSB-MP454885SYZ
CSB-EP454885SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Sulfate adenylyltransferase subunit 1 (cysN) CSB-YP454886SYZ
CSB-EP454886SYZ
CSB-BP454886SYZ
CSB-MP454886SYZ
CSB-EP454886SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 5'/3'-nucleotidase SurE (surE) CSB-YP454887SYZ
CSB-EP454887SYZ
CSB-BP454887SYZ
CSB-MP454887SYZ
CSB-EP454887SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0391 membrane protein ytjA (ytjA), partial CSB-YP454888SYZ
CSB-EP454888SYZ
CSB-BP454888SYZ
CSB-MP454888SYZ
CSB-EP454888SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Peptidyl-tRNA hydrolase (pth) CSB-YP454889SYZ
CSB-EP454889SYZ
CSB-BP454889SYZ
CSB-MP454889SYZ
CSB-EP454889SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0263 protein yciU (yciU) CSB-YP454890SYZ
CSB-EP454890SYZ
CSB-BP454890SYZ
CSB-MP454890SYZ
CSB-EP454890SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Regulatory protein recX (recX) CSB-YP454891SYZ
CSB-EP454891SYZ
CSB-BP454891SYZ
CSB-MP454891SYZ
CSB-EP454891SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP454892SYZ
CSB-EP454892SYZ
CSB-BP454892SYZ
CSB-MP454892SYZ
CSB-EP454892SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 UPF0283 membrane protein ycjF (ycjF), partial CSB-YP454893SYZ1
CSB-EP454893SYZ1
CSB-BP454893SYZ1
CSB-MP454893SYZ1
CSB-EP454893SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Protein sprT (sprT) CSB-YP454894SYZ
CSB-EP454894SYZ
CSB-BP454894SYZ
CSB-MP454894SYZ
CSB-EP454894SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Probable Fe (2+)-trafficking protein CSB-YP454895SYZ
CSB-EP454895SYZ
CSB-BP454895SYZ
CSB-MP454895SYZ
CSB-EP454895SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Multifunctional CCA protein (cca) CSB-YP454896SYZ
CSB-EP454896SYZ
CSB-BP454896SYZ
CSB-MP454896SYZ
CSB-EP454896SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 Spermidine export protein MdtJ (mdtJ), partial CSB-YP454897SYZ1
CSB-EP454897SYZ1
CSB-BP454897SYZ1
CSB-MP454897SYZ1
CSB-EP454897SYZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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