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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP455479RLV
CSB-EP455479RLV
CSB-BP455479RLV
CSB-MP455479RLV
CSB-EP455479RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Tryptophan synthase beta chain (trpB) CSB-YP455480RLV
CSB-EP455480RLV
CSB-BP455480RLV
CSB-MP455480RLV
CSB-EP455480RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP455481RLV
CSB-EP455481RLV
CSB-BP455481RLV
CSB-MP455481RLV
CSB-EP455481RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Serine--tRNA ligase (serS) CSB-YP455482RLV
CSB-EP455482RLV
CSB-BP455482RLV
CSB-MP455482RLV
CSB-EP455482RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris LexA repressor (lexA) CSB-YP455483RLV
CSB-EP455483RLV
CSB-BP455483RLV
CSB-MP455483RLV
CSB-EP455483RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit A (nuoA), partial CSB-YP455484RLV1
CSB-EP455484RLV1
CSB-BP455484RLV1
CSB-MP455484RLV1
CSB-EP455484RLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Putative NADH dehydrogenase/NAD (P)H nitroreductase Rpal_4764 CSB-YP455485RLV
CSB-EP455485RLV
CSB-BP455485RLV
CSB-MP455485RLV
CSB-EP455485RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP455486RLV
CSB-EP455486RLV
CSB-BP455486RLV
CSB-MP455486RLV
CSB-EP455486RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP455487RLV
CSB-EP455487RLV
CSB-BP455487RLV
CSB-MP455487RLV
CSB-EP455487RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Trigger factor (tig) CSB-YP455488RLV
CSB-EP455488RLV
CSB-BP455488RLV
CSB-MP455488RLV
CSB-EP455488RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP455489RLV
CSB-EP455489RLV
CSB-BP455489RLV
CSB-MP455489RLV
CSB-EP455489RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP455490RLV
CSB-EP455490RLV
CSB-BP455490RLV
CSB-MP455490RLV
CSB-EP455490RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribonuclease PH (rph) CSB-YP455491RLV
CSB-EP455491RLV
CSB-BP455491RLV
CSB-MP455491RLV
CSB-EP455491RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP455492RLV
CSB-EP455492RLV
CSB-BP455492RLV
CSB-MP455492RLV
CSB-EP455492RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP455493RLV
CSB-EP455493RLV
CSB-BP455493RLV
CSB-MP455493RLV
CSB-EP455493RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0061 protein Rpal_3454 (Rpal_3454) CSB-YP455494RLV
CSB-EP455494RLV
CSB-BP455494RLV
CSB-MP455494RLV
CSB-EP455494RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S6 (rpsF) CSB-YP455495RLV
CSB-EP455495RLV
CSB-BP455495RLV
CSB-MP455495RLV
CSB-EP455495RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP455496RLV
CSB-EP455496RLV
CSB-BP455496RLV
CSB-MP455496RLV
CSB-EP455496RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Acetate kinase (ackA) CSB-YP455497RLV
CSB-EP455497RLV
CSB-BP455497RLV
CSB-MP455497RLV
CSB-EP455497RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Catalase-peroxidase (katG), partial CSB-YP455498RLV
CSB-EP455498RLV
CSB-BP455498RLV
CSB-MP455498RLV
CSB-EP455498RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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