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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhodopseudomonas palustris Octanoyltransferase (lipB) CSB-YP455499RLV
CSB-EP455499RLV
CSB-BP455499RLV
CSB-MP455499RLV
CSB-EP455499RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP455500RLV
CSB-EP455500RLV
CSB-BP455500RLV
CSB-MP455500RLV
CSB-EP455500RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Coenzyme PQQ synthesis protein B (pqqB) CSB-YP455501RLV
CSB-EP455501RLV
CSB-BP455501RLV
CSB-MP455501RLV
CSB-EP455501RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 50S ribosomal protein L14 (rplN) CSB-YP455502RLV
CSB-EP455502RLV
CSB-BP455502RLV
CSB-MP455502RLV
CSB-EP455502RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 30S ribosomal protein S3 (rpsC) CSB-YP455503RLV
CSB-EP455503RLV
CSB-BP455503RLV
CSB-MP455503RLV
CSB-EP455503RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UPF0133 protein Rpal_0620 (Rpal_0620) CSB-YP455504RLV
CSB-EP455504RLV
CSB-BP455504RLV
CSB-MP455504RLV
CSB-EP455504RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP455505RLV
CSB-EP455505RLV
CSB-BP455505RLV
CSB-MP455505RLV
CSB-EP455505RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP455506RLV
CSB-EP455506RLV
CSB-BP455506RLV
CSB-MP455506RLV
CSB-EP455506RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris ATP phosphoribosyltransferase (hisG) CSB-YP455507RLV
CSB-EP455507RLV
CSB-BP455507RLV
CSB-MP455507RLV
CSB-EP455507RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP455508RLV
CSB-EP455508RLV
CSB-BP455508RLV
CSB-MP455508RLV
CSB-EP455508RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Ferredoxin--NADP reductase (Rpal_4475) CSB-YP455509RLV
CSB-EP455509RLV
CSB-BP455509RLV
CSB-MP455509RLV
CSB-EP455509RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris DNA repair protein recO (recO) CSB-YP455510RLV
CSB-EP455510RLV
CSB-BP455510RLV
CSB-MP455510RLV
CSB-EP455510RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Integration host factor subunit alpha (ihfA) CSB-YP455511RLV
CSB-EP455511RLV
CSB-BP455511RLV
CSB-MP455511RLV
CSB-EP455511RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Heme A synthase (ctaA), partial CSB-YP455512RLV
CSB-EP455512RLV
CSB-BP455512RLV
CSB-MP455512RLV
CSB-EP455512RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris 7-cyano-7-deazaguanine synthase (queC) CSB-YP455513RLV
CSB-EP455513RLV
CSB-BP455513RLV
CSB-MP455513RLV
CSB-EP455513RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP455514DSQ
CSB-EP455514DSQ
CSB-BP455514DSQ
CSB-MP455514DSQ
CSB-EP455514DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP455515DSQ
CSB-EP455515DSQ
CSB-BP455515DSQ
CSB-MP455515DSQ
CSB-EP455515DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP455516DSQ
CSB-EP455516DSQ
CSB-BP455516DSQ
CSB-MP455516DSQ
CSB-EP455516DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP455517DSQ
CSB-EP455517DSQ
CSB-BP455517DSQ
CSB-MP455517DSQ
CSB-EP455517DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobaculum parvum Probable pyruvoyl-dependent arginine decarboxylase (pdaD) CSB-YP455518DSQ
CSB-EP455518DSQ
CSB-BP455518DSQ
CSB-MP455518DSQ
CSB-EP455518DSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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