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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Chloroherpeton thalassium Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP455539DSY
CSB-EP455539DSY
CSB-BP455539DSY
CSB-MP455539DSY
CSB-EP455539DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Ketol-acid reductoisomerase (ilvC) CSB-YP455540DSY
CSB-EP455540DSY
CSB-BP455540DSY
CSB-MP455540DSY
CSB-EP455540DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Holo-[acyl-carrier-protein] synthase (acpS) CSB-YP455541DSY
CSB-EP455541DSY
CSB-BP455541DSY
CSB-MP455541DSY
CSB-EP455541DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Pyridoxine 5'-phosphate synthase (pdxJ) CSB-YP455542DSY
CSB-EP455542DSY
CSB-BP455542DSY
CSB-MP455542DSY
CSB-EP455542DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium D-alanine--D-alanine ligase (ddl) CSB-YP455543DSY
CSB-EP455543DSY
CSB-BP455543DSY
CSB-MP455543DSY
CSB-EP455543DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Ribosome maturation factor RimP (rimP) CSB-YP455544DSY
CSB-EP455544DSY
CSB-BP455544DSY
CSB-MP455544DSY
CSB-EP455544DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP455545DSY
CSB-EP455545DSY
CSB-BP455545DSY
CSB-MP455545DSY
CSB-EP455545DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Probable cytosol aminopeptidase (pepA), partial CSB-YP455546DSY
CSB-EP455546DSY
CSB-BP455546DSY
CSB-MP455546DSY
CSB-EP455546DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP455547DSY
CSB-EP455547DSY
CSB-BP455547DSY
CSB-MP455547DSY
CSB-EP455547DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium L-lactate dehydrogenase (ldh) CSB-YP455548DSY
CSB-EP455548DSY
CSB-BP455548DSY
CSB-MP455548DSY
CSB-EP455548DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Trigger factor (tig) CSB-YP455549DSY
CSB-EP455549DSY
CSB-BP455549DSY
CSB-MP455549DSY
CSB-EP455549DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 50S ribosomal protein L4 (rplD) CSB-YP455550DSY
CSB-EP455550DSY
CSB-BP455550DSY
CSB-MP455550DSY
CSB-EP455550DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium NADH-quinone oxidoreductase subunit D 2 (nuoD2) CSB-YP455551DSY
CSB-EP455551DSY
CSB-BP455551DSY
CSB-MP455551DSY
CSB-EP455551DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP455552DSY
CSB-EP455552DSY
CSB-BP455552DSY
CSB-MP455552DSY
CSB-EP455552DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 30S ribosomal protein S14 (rpsN) CSB-YP455553DSY
CSB-EP455553DSY
CSB-BP455553DSY
CSB-MP455553DSY
CSB-EP455553DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 50S ribosomal protein L36 (rpmJ) CSB-YP455554DSY
CSB-EP455554DSY
CSB-BP455554DSY
CSB-MP455554DSY
CSB-EP455554DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Recombination protein RecR (recR) CSB-YP455555DSY
CSB-EP455555DSY
CSB-BP455555DSY
CSB-MP455555DSY
CSB-EP455555DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Proline--tRNA ligase (proS) CSB-YP455556DSY
CSB-EP455556DSY
CSB-BP455556DSY
CSB-MP455556DSY
CSB-EP455556DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP455557DSY
CSB-EP455557DSY
CSB-BP455557DSY
CSB-MP455557DSY
CSB-EP455557DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloroherpeton thalassium Light-independent protochlorophyllide reductase subunit B (bchB), partial CSB-YP455558DSY
CSB-EP455558DSY
CSB-BP455558DSY
CSB-MP455558DSY
CSB-EP455558DSY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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