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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leptothrix cholodnii 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP455939LOT
CSB-EP455939LOT
CSB-BP455939LOT
CSB-MP455939LOT
CSB-EP455939LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP455940LOT
CSB-EP455940LOT
CSB-BP455940LOT
CSB-MP455940LOT
CSB-EP455940LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP455941LOT
CSB-EP455941LOT
CSB-BP455941LOT
CSB-MP455941LOT
CSB-EP455941LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S21 (rpsU) CSB-YP455942LOT
CSB-EP455942LOT
CSB-BP455942LOT
CSB-MP455942LOT
CSB-EP455942LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S16 (rpsP) CSB-YP455943LOT
CSB-EP455943LOT
CSB-BP455943LOT
CSB-MP455943LOT
CSB-EP455943LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP455944LOT
CSB-EP455944LOT
CSB-BP455944LOT
CSB-MP455944LOT
CSB-EP455944LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L28 (rpmB) CSB-YP455945LOT
CSB-EP455945LOT
CSB-BP455945LOT
CSB-MP455945LOT
CSB-EP455945LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP455946LOT
CSB-EP455946LOT
CSB-BP455946LOT
CSB-MP455946LOT
CSB-EP455946LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L21 (rplU) CSB-YP455947LOT
CSB-EP455947LOT
CSB-BP455947LOT
CSB-MP455947LOT
CSB-EP455947LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Probable Fe (2+)-trafficking protein CSB-YP455948LOT
CSB-EP455948LOT
CSB-BP455948LOT
CSB-MP455948LOT
CSB-EP455948LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP455949LOT
CSB-EP455949LOT
CSB-BP455949LOT
CSB-MP455949LOT
CSB-EP455949LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Urease accessory protein UreD (ureD) CSB-YP455950LOT
CSB-EP455950LOT
CSB-BP455950LOT
CSB-MP455950LOT
CSB-EP455950LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 8-amino-7-oxononanoate synthase (bioF) CSB-YP455951LOT
CSB-EP455951LOT
CSB-BP455951LOT
CSB-MP455951LOT
CSB-EP455951LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP455952LOT
CSB-EP455952LOT
CSB-BP455952LOT
CSB-MP455952LOT
CSB-EP455952LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) CSB-YP455953LOT
CSB-EP455953LOT
CSB-BP455953LOT
CSB-MP455953LOT
CSB-EP455953LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Adenylate kinase (adk) CSB-YP455954LOT
CSB-EP455954LOT
CSB-BP455954LOT
CSB-MP455954LOT
CSB-EP455954LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Nitrogenase-stabilizing/protective protein nifW (nifW) CSB-YP455955LOT
CSB-EP455955LOT
CSB-BP455955LOT
CSB-MP455955LOT
CSB-EP455955LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S10 (rpsJ) CSB-YP455956LOT
CSB-EP455956LOT
CSB-BP455956LOT
CSB-MP455956LOT
CSB-EP455956LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP455957LOT
CSB-EP455957LOT
CSB-BP455957LOT
CSB-MP455957LOT
CSB-EP455957LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii Dihydroxy-acid dehydratase (ilvD), partial CSB-YP455958LOT
CSB-EP455958LOT
CSB-BP455958LOT
CSB-MP455958LOT
CSB-EP455958LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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