Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ralstonia pickettii Cobalamin synthase (cobS), partial CSB-YP456840RJI1
CSB-EP456840RJI1
CSB-BP456840RJI1
CSB-MP456840RJI1
CSB-EP456840RJI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Deoxyuridine 5'-triphosphate nucleotidohydrolase (dut) CSB-YP456841RJI
CSB-EP456841RJI
CSB-BP456841RJI
CSB-MP456841RJI
CSB-EP456841RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii DNA ligase (ligA), partial CSB-YP456842RJI
CSB-EP456842RJI
CSB-BP456842RJI
CSB-MP456842RJI
CSB-EP456842RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii UPF0234 protein Rpic_2826 (Rpic_2826) CSB-YP456843RJI
CSB-EP456843RJI
CSB-BP456843RJI
CSB-MP456843RJI
CSB-EP456843RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) CSB-YP456844RJI
CSB-EP456844RJI
CSB-BP456844RJI
CSB-MP456844RJI
CSB-EP456844RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP456845RJI
CSB-EP456845RJI
CSB-BP456845RJI
CSB-MP456845RJI
CSB-EP456845RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP456846RJI
CSB-EP456846RJI
CSB-BP456846RJI
CSB-MP456846RJI
CSB-EP456846RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Glutamate 5-kinase (proB) CSB-YP456847RJI
CSB-EP456847RJI
CSB-BP456847RJI
CSB-MP456847RJI
CSB-EP456847RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP456848RJI
CSB-EP456848RJI
CSB-BP456848RJI
CSB-MP456848RJI
CSB-EP456848RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP456849RJI
CSB-EP456849RJI
CSB-BP456849RJI
CSB-MP456849RJI
CSB-EP456849RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP456850RJI
CSB-EP456850RJI
CSB-BP456850RJI
CSB-MP456850RJI
CSB-EP456850RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Protein-export protein secB (secB) CSB-YP456851RJI
CSB-EP456851RJI
CSB-BP456851RJI
CSB-MP456851RJI
CSB-EP456851RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 30S ribosomal protein S13 (rpsM) CSB-YP456852RJI
CSB-EP456852RJI
CSB-BP456852RJI
CSB-MP456852RJI
CSB-EP456852RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 30S ribosomal protein S17 (rpsQ) CSB-YP456853RJI
CSB-EP456853RJI
CSB-BP456853RJI
CSB-MP456853RJI
CSB-EP456853RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP456854RJI
CSB-EP456854RJI
CSB-BP456854RJI
CSB-MP456854RJI
CSB-EP456854RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP456855RJI
CSB-EP456855RJI
CSB-BP456855RJI
CSB-MP456855RJI
CSB-EP456855RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii 50S ribosomal protein L1 (rplA) CSB-YP456856RJI
CSB-EP456856RJI
CSB-BP456856RJI
CSB-MP456856RJI
CSB-EP456856RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP456857RJI
CSB-EP456857RJI
CSB-BP456857RJI
CSB-MP456857RJI
CSB-EP456857RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Regulatory protein recX (recX) CSB-YP456858RJI
CSB-EP456858RJI
CSB-BP456858RJI
CSB-MP456858RJI
CSB-EP456858RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia pickettii Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP456859RJI
CSB-EP456859RJI
CSB-BP456859RJI
CSB-MP456859RJI
CSB-EP456859RJI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>