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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Leptothrix cholodnii NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP455959LOT
CSB-EP455959LOT
CSB-BP455959LOT
CSB-MP455959LOT
CSB-EP455959LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S11 (rpsK) CSB-YP455960LOT
CSB-EP455960LOT
CSB-BP455960LOT
CSB-MP455960LOT
CSB-EP455960LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L6 (rplF) CSB-YP455961LOT
CSB-EP455961LOT
CSB-BP455961LOT
CSB-MP455961LOT
CSB-EP455961LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 30S ribosomal protein S17 (rpsQ) CSB-YP455962LOT
CSB-EP455962LOT
CSB-BP455962LOT
CSB-MP455962LOT
CSB-EP455962LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptothrix cholodnii 50S ribosomal protein L4 (rplD) CSB-YP455963LOT
CSB-EP455963LOT
CSB-BP455963LOT
CSB-MP455963LOT
CSB-EP455963LOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L18P (rpl18p) CSB-YP455964TNE
CSB-EP455964TNE
CSB-BP455964TNE
CSB-MP455964TNE
CSB-EP455964TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Proline--tRNA ligase (proS) CSB-YP455965TNE
CSB-EP455965TNE
CSB-BP455965TNE
CSB-MP455965TNE
CSB-EP455965TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) CSB-YP455966TNE
CSB-EP455966TNE
CSB-BP455966TNE
CSB-MP455966TNE
CSB-EP455966TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Ketol-acid reductoisomerase (ilvC) CSB-YP455967TNE
CSB-EP455967TNE
CSB-BP455967TNE
CSB-MP455967TNE
CSB-EP455967TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L24e (rpl24e) CSB-YP455968TNE
CSB-EP455968TNE
CSB-BP455968TNE
CSB-MP455968TNE
CSB-EP455968TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Probable tRNA pseudouridine synthase B (truB) CSB-YP455969TNE
CSB-EP455969TNE
CSB-BP455969TNE
CSB-MP455969TNE
CSB-EP455969TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Glutamyl-tRNA (Gln) amidotransferase subunit E, partial CSB-YP455970TNE
CSB-EP455970TNE
CSB-BP455970TNE
CSB-MP455970TNE
CSB-EP455970TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus N-acetyl-gamma-glutamyl-phosphate/N-acetyl-gamma-aminoadipyl-phosphate reductase (argC) CSB-YP455971TNE
CSB-EP455971TNE
CSB-BP455971TNE
CSB-MP455971TNE
CSB-EP455971TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Indole-3-glycerol phosphate synthase (trpC) CSB-YP455972TNE
CSB-EP455972TNE
CSB-BP455972TNE
CSB-MP455972TNE
CSB-EP455972TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus V-type ATP synthase alpha chain (atpA), partial CSB-YP455973TNE
CSB-EP455973TNE
CSB-BP455973TNE
CSB-MP455973TNE
CSB-EP455973TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Chromatin protein Cren7 (creN7) CSB-YP455974TNE
CSB-EP455974TNE
CSB-BP455974TNE
CSB-MP455974TNE
CSB-EP455974TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP455975TNE
CSB-EP455975TNE
CSB-BP455975TNE
CSB-MP455975TNE
CSB-EP455975TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus 50S ribosomal protein L3P (rpl3p) CSB-YP455976TNE
CSB-EP455976TNE
CSB-BP455976TNE
CSB-MP455976TNE
CSB-EP455976TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermoproteus neutrophilus Proteasome subunit beta 2 (psmB2) CSB-YP455977TNE
CSB-EP455977TNE
CSB-BP455977TNE
CSB-MP455977TNE
CSB-EP455977TNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exiguobacterium sibiricum LexA repressor (lexA) CSB-YP455978EPT
CSB-EP455978EPT
CSB-BP455978EPT
CSB-MP455978EPT
CSB-EP455978EPT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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