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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia tasmaniensis Succinylglutamate desuccinylase (astE) CSB-YP456980ENB
CSB-EP456980ENB
CSB-BP456980ENB
CSB-MP456980ENB
CSB-EP456980ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L20 (rplT) CSB-YP456981ENB
CSB-EP456981ENB
CSB-BP456981ENB
CSB-MP456981ENB
CSB-EP456981ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Electron transport complex protein RnfE (rnfE), partial CSB-YP456982ENB1
CSB-EP456982ENB1
CSB-BP456982ENB1
CSB-MP456982ENB1
CSB-EP456982ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0756 membrane protein ETA_17460 (ETA_17460), partial CSB-YP456983ENB1
CSB-EP456983ENB1
CSB-BP456983ENB1
CSB-MP456983ENB1
CSB-EP456983ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis UPF0301 protein ETA_28320 (ETA_28320) CSB-YP456984ENB
CSB-EP456984ENB
CSB-BP456984ENB
CSB-MP456984ENB
CSB-EP456984ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Cell division protein ZapA (zapA) CSB-YP456985ENB
CSB-EP456985ENB
CSB-BP456985ENB
CSB-MP456985ENB
CSB-EP456985ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP456986ENB
CSB-EP456986ENB
CSB-BP456986ENB
CSB-MP456986ENB
CSB-EP456986ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Catalase-peroxidase (katG), partial CSB-YP456987ENB
CSB-EP456987ENB
CSB-BP456987ENB
CSB-MP456987ENB
CSB-EP456987ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Enolase (eno) CSB-YP456988ENB
CSB-EP456988ENB
CSB-BP456988ENB
CSB-MP456988ENB
CSB-EP456988ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis DNA mismatch repair protein MutS (mutS), partial CSB-YP456989ENB
CSB-EP456989ENB
CSB-BP456989ENB
CSB-MP456989ENB
CSB-EP456989ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Tat-linked quality control protein TatD (tatD) CSB-YP456990ENB
CSB-EP456990ENB
CSB-BP456990ENB
CSB-MP456990ENB
CSB-EP456990ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) CSB-YP456991ENB
CSB-EP456991ENB
CSB-BP456991ENB
CSB-MP456991ENB
CSB-EP456991ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 30S ribosomal protein S21 (rpsU) CSB-YP456992ENB
CSB-EP456992ENB
CSB-BP456992ENB
CSB-MP456992ENB
CSB-EP456992ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Polyribonucleotide nucleotidyltransferase (pnp), partial CSB-YP456993ENB
CSB-EP456993ENB
CSB-BP456993ENB
CSB-MP456993ENB
CSB-EP456993ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L21 (rplU) CSB-YP456994ENB
CSB-EP456994ENB
CSB-BP456994ENB
CSB-MP456994ENB
CSB-EP456994ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA), partial CSB-YP456995ENB1
CSB-EP456995ENB1
CSB-BP456995ENB1
CSB-MP456995ENB1
CSB-EP456995ENB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Probable phosphoglycerate mutase GpmB (gpmB) CSB-YP456996ENB
CSB-EP456996ENB
CSB-BP456996ENB
CSB-MP456996ENB
CSB-EP456996ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Deoxyribose-phosphate aldolase (deoC) CSB-YP456997ENB
CSB-EP456997ENB
CSB-BP456997ENB
CSB-MP456997ENB
CSB-EP456997ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis 50S ribosomal protein L19 (rplS) CSB-YP456998ENB
CSB-EP456998ENB
CSB-BP456998ENB
CSB-MP456998ENB
CSB-EP456998ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP456999ENB
CSB-EP456999ENB
CSB-BP456999ENB
CSB-MP456999ENB
CSB-EP456999ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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