Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Chaperone protein hscA homolog (hscA), partial CSB-YP461121AXI
CSB-EP461121AXI
CSB-BP461121AXI
CSB-MP461121AXI
CSB-EP461121AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L31 (rpmE) CSB-YP461122AXI
CSB-EP461122AXI
CSB-BP461122AXI
CSB-MP461122AXI
CSB-EP461122AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Regulatory protein recX (recX) CSB-YP461123AXI
CSB-EP461123AXI
CSB-BP461123AXI
CSB-MP461123AXI
CSB-EP461123AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP461124AXI
CSB-EP461124AXI
CSB-BP461124AXI
CSB-MP461124AXI
CSB-EP461124AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0102 protein APP7_1414 (APP7_1414) CSB-YP461125AXI
CSB-EP461125AXI
CSB-BP461125AXI
CSB-MP461125AXI
CSB-EP461125AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Trigger factor (tig) CSB-YP461126AXI
CSB-EP461126AXI
CSB-BP461126AXI
CSB-MP461126AXI
CSB-EP461126AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 tRNA (Ile)-lysidine synthase CSB-YP461127AXI
CSB-EP461127AXI
CSB-BP461127AXI
CSB-MP461127AXI
CSB-EP461127AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L1 (rplA) CSB-YP461128AXI
CSB-EP461128AXI
CSB-BP461128AXI
CSB-MP461128AXI
CSB-EP461128AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Probable Fe (2+)-trafficking protein CSB-YP461129AXI
CSB-EP461129AXI
CSB-BP461129AXI
CSB-MP461129AXI
CSB-EP461129AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP461130AXI
CSB-EP461130AXI
CSB-BP461130AXI
CSB-MP461130AXI
CSB-EP461130AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S10 (rpsJ) CSB-YP461131AXI
CSB-EP461131AXI
CSB-BP461131AXI
CSB-MP461131AXI
CSB-EP461131AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S14 (rpsN) CSB-YP461132AXI
CSB-EP461132AXI
CSB-BP461132AXI
CSB-MP461132AXI
CSB-EP461132AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S4 (rpsD) CSB-YP461133AXI
CSB-EP461133AXI
CSB-BP461133AXI
CSB-MP461133AXI
CSB-EP461133AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L19 (rplS) CSB-YP461134AXI
CSB-EP461134AXI
CSB-BP461134AXI
CSB-MP461134AXI
CSB-EP461134AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP461135AXI
CSB-EP461135AXI
CSB-BP461135AXI
CSB-MP461135AXI
CSB-EP461135AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP461136AXI
CSB-EP461136AXI
CSB-BP461136AXI
CSB-MP461136AXI
CSB-EP461136AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 DNA replication and repair protein recF (recF) CSB-YP461137AXI
CSB-EP461137AXI
CSB-BP461137AXI
CSB-MP461137AXI
CSB-EP461137AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0133 protein APP7_0075 (APP7_0075) CSB-YP461138AXI
CSB-EP461138AXI
CSB-BP461138AXI
CSB-MP461138AXI
CSB-EP461138AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Na (+)-translocating NADH-quinone reductase subunit A CSB-YP461139AXI
CSB-EP461139AXI
CSB-BP461139AXI
CSB-MP461139AXI
CSB-EP461139AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Putative Holliday junction resolvase (APP7_0233) CSB-YP461140AXI
CSB-EP461140AXI
CSB-BP461140AXI
CSB-MP461140AXI
CSB-EP461140AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>