Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus pleuropneumoniae serotype 7 Orotate phosphoribosyltransferase (pyrE) CSB-YP461141AXI
CSB-EP461141AXI
CSB-BP461141AXI
CSB-MP461141AXI
CSB-EP461141AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP461142AXI
CSB-EP461142AXI
CSB-BP461142AXI
CSB-MP461142AXI
CSB-EP461142AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Uracil phosphoribosyltransferase (upp) CSB-YP461143AXI
CSB-EP461143AXI
CSB-BP461143AXI
CSB-MP461143AXI
CSB-EP461143AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 50S ribosomal protein L13 (rplM) CSB-YP461144AXI
CSB-EP461144AXI
CSB-BP461144AXI
CSB-MP461144AXI
CSB-EP461144AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Integration host factor subunit alpha (ihfA) CSB-YP461145AXI
CSB-EP461145AXI
CSB-BP461145AXI
CSB-MP461145AXI
CSB-EP461145AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Elongation factor P (efp) CSB-YP461146AXI
CSB-EP461146AXI
CSB-BP461146AXI
CSB-MP461146AXI
CSB-EP461146AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP461147AXI
CSB-EP461147AXI
CSB-BP461147AXI
CSB-MP461147AXI
CSB-EP461147AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Lysine--tRNA ligase (lysS) CSB-YP461148AXI
CSB-EP461148AXI
CSB-BP461148AXI
CSB-MP461148AXI
CSB-EP461148AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 UPF0181 protein APP7_0922 (APP7_0922) CSB-YP461149AXI
CSB-EP461149AXI
CSB-BP461149AXI
CSB-MP461149AXI
CSB-EP461149AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP461150AXI
CSB-EP461150AXI
CSB-BP461150AXI
CSB-MP461150AXI
CSB-EP461150AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 7-cyano-7-deazaguanine synthase (queC) CSB-YP461151AXI
CSB-EP461151AXI
CSB-BP461151AXI
CSB-MP461151AXI
CSB-EP461151AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Glutamate--tRNA ligase (gltX) CSB-YP461152AXI
CSB-EP461152AXI
CSB-BP461152AXI
CSB-MP461152AXI
CSB-EP461152AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Cell division protein ZipA homolog (zipA), partial CSB-YP461153AXI
CSB-EP461153AXI
CSB-BP461153AXI
CSB-MP461153AXI
CSB-EP461153AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 30S ribosomal protein S21 (rpsU) CSB-YP461154AXI
CSB-EP461154AXI
CSB-BP461154AXI
CSB-MP461154AXI
CSB-EP461154AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Probable malate:quinone oxidoreductase (mqo) CSB-YP461155AXI
CSB-EP461155AXI
CSB-BP461155AXI
CSB-MP461155AXI
CSB-EP461155AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP461156AXI
CSB-EP461156AXI
CSB-BP461156AXI
CSB-MP461156AXI
CSB-EP461156AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Urease subunit gamma (ureA) CSB-YP461157AXI
CSB-EP461157AXI
CSB-BP461157AXI
CSB-MP461157AXI
CSB-EP461157AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP461158AXI
CSB-EP461158AXI
CSB-BP461158AXI
CSB-MP461158AXI
CSB-EP461158AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Chaperone protein DnaK (dnaK), partial CSB-YP461159AXI
CSB-EP461159AXI
CSB-BP461159AXI
CSB-MP461159AXI
CSB-EP461159AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 D-tyrosyl-tRNA (Tyr) deacylase CSB-YP461160AXI
CSB-EP461160AXI
CSB-BP461160AXI
CSB-MP461160AXI
CSB-EP461160AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>