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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aquareovirus G Non-structural protein 4 (S7) CSB-YP461942DNU
CSB-EP461942DNU
CSB-BP461942DNU
CSB-MP461942DNU
CSB-EP461942DNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus caracteristicus Conotoxin Ca8.3 CSB-YP461943DVW
CSB-EP461943DVW
CSB-BP461943DVW
CSB-MP461943DVW
CSB-EP461943DVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phomopsis amygdali Geranylgeranyl pyrophosphate synthase A (GGS-A) CSB-YP461944EUU
CSB-EP461944EUU
CSB-BP461944EUU
CSB-MP461944EUU
CSB-EP461944EUU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodotorula glutinis ATPase GET3 (GET3) CSB-YP461945RME
CSB-EP461945RME
CSB-BP461945RME
CSB-MP461945RME
CSB-EP461945RME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP461946FLX
CSB-EP461946FLX
CSB-BP461946FLX
CSB-MP461946FLX
CSB-EP461946FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia D-tyrosyl-tRNA (Tyr) deacylase CSB-YP461947FLX
CSB-EP461947FLX
CSB-BP461947FLX
CSB-MP461947FLX
CSB-EP461947FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Type III pantothenate kinase (coaX) CSB-YP461948FLX
CSB-EP461948FLX
CSB-BP461948FLX
CSB-MP461948FLX
CSB-EP461948FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 3-dehydroquinate dehydratase (aroQ) CSB-YP461949FLX
CSB-EP461949FLX
CSB-BP461949FLX
CSB-MP461949FLX
CSB-EP461949FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia RNA pyrophosphohydrolase (rppH) CSB-YP461950FLX
CSB-EP461950FLX
CSB-BP461950FLX
CSB-MP461950FLX
CSB-EP461950FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Orotate phosphoribosyltransferase (pyrE) CSB-YP461951FLX
CSB-EP461951FLX
CSB-BP461951FLX
CSB-MP461951FLX
CSB-EP461951FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Cell division protein FtsB (ftsB), partial CSB-YP461952FLX
CSB-EP461952FLX
CSB-BP461952FLX
CSB-MP461952FLX
CSB-EP461952FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia FMN-dependent NADH-azoreductase (azoR) CSB-YP461953FLX
CSB-EP461953FLX
CSB-BP461953FLX
CSB-MP461953FLX
CSB-EP461953FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Putative NADH dehydrogenase/NAD (P)H nitroreductase Smlt0482 CSB-YP461954FLX
CSB-EP461954FLX
CSB-BP461954FLX
CSB-MP461954FLX
CSB-EP461954FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP461955FLX
CSB-EP461955FLX
CSB-BP461955FLX
CSB-MP461955FLX
CSB-EP461955FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 8-amino-7-oxononanoate synthase (bioF) CSB-YP461956FLX
CSB-EP461956FLX
CSB-BP461956FLX
CSB-MP461956FLX
CSB-EP461956FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Putative pterin-4-alpha-carbinolamine dehydratase (Smlt3243) CSB-YP461957FLX
CSB-EP461957FLX
CSB-BP461957FLX
CSB-MP461957FLX
CSB-EP461957FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Dihydroxy-acid dehydratase (ilvD), partial CSB-YP461958FLX
CSB-EP461958FLX
CSB-BP461958FLX
CSB-MP461958FLX
CSB-EP461958FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia SsrA-binding protein (smpB) CSB-YP461959FLX
CSB-EP461959FLX
CSB-BP461959FLX
CSB-MP461959FLX
CSB-EP461959FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP461960FLX
CSB-EP461960FLX
CSB-BP461960FLX
CSB-MP461960FLX
CSB-EP461960FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Coproporphyrinogen-III oxidase, aerobic (hemF) CSB-YP461961FLX
CSB-EP461961FLX
CSB-BP461961FLX
CSB-MP461961FLX
CSB-EP461961FLX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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