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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pneumoniae Chromosomal replication initiator protein DnaA (dnaA) CSB-YP462202FMZ
CSB-EP462202FMZ
CSB-BP462202FMZ
CSB-MP462202FMZ
CSB-EP462202FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae UPF0042 nucleotide-binding protein SPCG_1551 (SPCG_1551) CSB-YP462203FMZ
CSB-EP462203FMZ
CSB-BP462203FMZ
CSB-MP462203FMZ
CSB-EP462203FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP462204FMZ
CSB-EP462204FMZ
CSB-BP462204FMZ
CSB-MP462204FMZ
CSB-EP462204FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae D-alanine--D-alanine ligase (ddl) CSB-YP462205FMZ
CSB-EP462205FMZ
CSB-BP462205FMZ
CSB-MP462205FMZ
CSB-EP462205FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP462206FMZ
CSB-EP462206FMZ
CSB-BP462206FMZ
CSB-MP462206FMZ
CSB-EP462206FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 30S ribosomal protein S3 (rpsC) CSB-YP462207FMZ
CSB-EP462207FMZ
CSB-BP462207FMZ
CSB-MP462207FMZ
CSB-EP462207FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae 50S ribosomal protein L14 (rplN) CSB-YP462208FMZ
CSB-EP462208FMZ
CSB-BP462208FMZ
CSB-MP462208FMZ
CSB-EP462208FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Leucine--tRNA ligase (leuS), partial CSB-YP462209FMZ
CSB-EP462209FMZ
CSB-BP462209FMZ
CSB-MP462209FMZ
CSB-EP462209FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Xanthine phosphoribosyltransferase (xpt) CSB-YP462210FMZ
CSB-EP462210FMZ
CSB-BP462210FMZ
CSB-MP462210FMZ
CSB-EP462210FMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L32 (rpmF) CSB-YP462211NHQ
CSB-EP462211NHQ
CSB-BP462211NHQ
CSB-MP462211NHQ
CSB-EP462211NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L31 (rpmE) CSB-YP462212NHQ
CSB-EP462212NHQ
CSB-BP462212NHQ
CSB-MP462212NHQ
CSB-EP462212NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L18 (rplR) CSB-YP462213NHQ
CSB-EP462213NHQ
CSB-BP462213NHQ
CSB-MP462213NHQ
CSB-EP462213NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L24 (rplX) CSB-YP462214NHQ
CSB-EP462214NHQ
CSB-BP462214NHQ
CSB-MP462214NHQ
CSB-EP462214NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme 50S ribosomal protein L22 (rplV) CSB-YP462215NHQ
CSB-EP462215NHQ
CSB-BP462215NHQ
CSB-MP462215NHQ
CSB-EP462215NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme NAD (P)H-quinone oxidoreductase subunit N CSB-YP462216NHQ
CSB-EP462216NHQ
CSB-BP462216NHQ
CSB-MP462216NHQ
CSB-EP462216NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP462217NHQ
CSB-EP462217NHQ
CSB-BP462217NHQ
CSB-MP462217NHQ
CSB-EP462217NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Protein translocase subunit SecA (secA), partial CSB-YP462218NHQ
CSB-EP462218NHQ
CSB-BP462218NHQ
CSB-MP462218NHQ
CSB-EP462218NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme UDP-3-O-acylglucosamine N-acyltransferase (lpxD) CSB-YP462219NHQ
CSB-EP462219NHQ
CSB-BP462219NHQ
CSB-MP462219NHQ
CSB-EP462219NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Cytochrome c-550 (psbV) CSB-YP462220NHQ
CSB-EP462220NHQ
CSB-BP462220NHQ
CSB-MP462220NHQ
CSB-EP462220NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc punctiforme Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP462221NHQ
CSB-EP462221NHQ
CSB-BP462221NHQ
CSB-MP462221NHQ
CSB-EP462221NHQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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