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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus Glycerol kinase (glpK) CSB-YP472345BQP
CSB-EP472345BQP
CSB-BP472345BQP
CSB-MP472345BQP
CSB-EP472345BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0354 protein BCG9842_B0431 (BCG9842_B0431) CSB-YP472346BQP
CSB-EP472346BQP
CSB-BP472346BQP
CSB-MP472346BQP
CSB-EP472346BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP472347BQP
CSB-EP472347BQP
CSB-BP472347BQP
CSB-MP472347BQP
CSB-EP472347BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Bis (5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] CSB-YP472348BQP
CSB-EP472348BQP
CSB-BP472348BQP
CSB-MP472348BQP
CSB-EP472348BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0477 protein BCG9842_B4061 (BCG9842_B4061) CSB-YP472349BQP
CSB-EP472349BQP
CSB-BP472349BQP
CSB-MP472349BQP
CSB-EP472349BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Probable cytosol aminopeptidase (pepA) CSB-YP472350BQP
CSB-EP472350BQP
CSB-BP472350BQP
CSB-MP472350BQP
CSB-EP472350BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP472351BQP
CSB-EP472351BQP
CSB-BP472351BQP
CSB-MP472351BQP
CSB-EP472351BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus ATP phosphoribosyltransferase (hisG) CSB-YP472352BQP
CSB-EP472352BQP
CSB-BP472352BQP
CSB-MP472352BQP
CSB-EP472352BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP472353BQP
CSB-EP472353BQP
CSB-BP472353BQP
CSB-MP472353BQP
CSB-EP472353BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Cytidylate kinase (cmk) CSB-YP472354BQP
CSB-EP472354BQP
CSB-BP472354BQP
CSB-MP472354BQP
CSB-EP472354BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Dihydrodipicolinate reductase (dapB) CSB-YP472355BQP
CSB-EP472355BQP
CSB-BP472355BQP
CSB-MP472355BQP
CSB-EP472355BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Holliday junction resolvase recU (recU) CSB-YP472356BQP
CSB-EP472356BQP
CSB-BP472356BQP
CSB-MP472356BQP
CSB-EP472356BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0303 protein BCG9842_B1842 (BCG9842_B1842) CSB-YP472357BQP
CSB-EP472357BQP
CSB-BP472357BQP
CSB-MP472357BQP
CSB-EP472357BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphomethylpyrimidine synthase (thiC), partial CSB-YP472358BQP
CSB-EP472358BQP
CSB-BP472358BQP
CSB-MP472358BQP
CSB-EP472358BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP472359BQP
CSB-EP472359BQP
CSB-BP472359BQP
CSB-MP472359BQP
CSB-EP472359BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP472360BQP1
CSB-EP472360BQP1
CSB-BP472360BQP1
CSB-MP472360BQP1
CSB-EP472360BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgA (lrgA), partial CSB-YP472361BQP1
CSB-EP472361BQP1
CSB-BP472361BQP1
CSB-MP472361BQP1
CSB-EP472361BQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S6 (rpsF) CSB-YP472362BQP
CSB-EP472362BQP
CSB-BP472362BQP
CSB-MP472362BQP
CSB-EP472362BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0133 protein BCG9842_B5293 (BCG9842_B5293) CSB-YP472363BQP
CSB-EP472363BQP
CSB-BP472363BQP
CSB-MP472363BQP
CSB-EP472363BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Small, acid-soluble spore protein O (sspO) CSB-YP472364BQP
CSB-EP472364BQP
CSB-BP472364BQP
CSB-MP472364BQP
CSB-EP472364BQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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