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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus cereus NADH-quinone oxidoreductase subunit K (nuoK), partial CSB-YP476546BQM1
CSB-EP476546BQM1
CSB-BP476546BQM1
CSB-MP476546BQM1
CSB-EP476546BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0059 membrane protein BCAH820_5416 (BCAH820_5416), partial CSB-YP476547BQM1
CSB-EP476547BQM1
CSB-BP476547BQM1
CSB-MP476547BQM1
CSB-EP476547BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 3-dehydroquinate synthase (aroB) CSB-YP476548BQM
CSB-EP476548BQM
CSB-BP476548BQM
CSB-MP476548BQM
CSB-EP476548BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus CCA-adding enzyme (cca) CSB-YP476549BQM
CSB-EP476549BQM
CSB-BP476549BQM
CSB-MP476549BQM
CSB-EP476549BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0447 protein BCAH820_5483 (BCAH820_5483) CSB-YP476550BQM
CSB-EP476550BQM
CSB-BP476550BQM
CSB-MP476550BQM
CSB-EP476550BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0398 protein BCAH820_1652 (BCAH820_1652) CSB-YP476551BQM
CSB-EP476551BQM
CSB-BP476551BQM
CSB-MP476551BQM
CSB-EP476551BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Antiholin-like protein LrgB (lrgB), partial CSB-YP476552BQM1
CSB-EP476552BQM1
CSB-BP476552BQM1
CSB-MP476552BQM1
CSB-EP476552BQM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L34 (rpmH) CSB-YP476553BQM
CSB-EP476553BQM
CSB-BP476553BQM
CSB-MP476553BQM
CSB-EP476553BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Putative competence-damage inducible protein (cinA) CSB-YP476554BQM
CSB-EP476554BQM
CSB-BP476554BQM
CSB-MP476554BQM
CSB-EP476554BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Proline--tRNA ligase (proS) CSB-YP476555BQM
CSB-EP476555BQM
CSB-BP476555BQM
CSB-MP476555BQM
CSB-EP476555BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S2 (rpsB) CSB-YP476556BQM
CSB-EP476556BQM
CSB-BP476556BQM
CSB-MP476556BQM
CSB-EP476556BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP476557BQM
CSB-EP476557BQM
CSB-BP476557BQM
CSB-MP476557BQM
CSB-EP476557BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 30S ribosomal protein S16 (rpsP) CSB-YP476558BQM
CSB-EP476558BQM
CSB-BP476558BQM
CSB-MP476558BQM
CSB-EP476558BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Phosphate acyltransferase (plsX) CSB-YP476559BQM
CSB-EP476559BQM
CSB-BP476559BQM
CSB-MP476559BQM
CSB-EP476559BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Orotate phosphoribosyltransferase (pyrE) CSB-YP476560BQM
CSB-EP476560BQM
CSB-BP476560BQM
CSB-MP476560BQM
CSB-EP476560BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Aspartate carbamoyltransferase (pyrB) CSB-YP476561BQM
CSB-EP476561BQM
CSB-BP476561BQM
CSB-MP476561BQM
CSB-EP476561BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus Isoleucine--tRNA ligase (ileS) CSB-YP476562BQM
CSB-EP476562BQM
CSB-BP476562BQM
CSB-MP476562BQM
CSB-EP476562BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus 50S ribosomal protein L32 (rpmF) CSB-YP476563BQM
CSB-EP476563BQM
CSB-BP476563BQM
CSB-MP476563BQM
CSB-EP476563BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus UPF0358 protein BCAH820_3964 (BCAH820_3964) CSB-YP476564BQM
CSB-EP476564BQM
CSB-BP476564BQM
CSB-MP476564BQM
CSB-EP476564BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus cereus DNA integrity scanning protein DisA (disA) CSB-YP476565BQM
CSB-EP476565BQM
CSB-BP476565BQM
CSB-MP476565BQM
CSB-EP476565BQM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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