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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Balaenoptera musculus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078BEC1
CSB-EP015078BEC1
CSB-BP015078BEC1
CSB-MP015078BEC1
CSB-EP015078BEC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tupaia glis Alpha-crystallin A chain (CRYAA) CSB-YP006007TJH
CSB-EP006007TJH
CSB-BP006007TJH
CSB-MP006007TJH
CSB-EP006007TJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss ATP synthase protein 8 (mt-atp8), partial CSB-YP015071OEI
CSB-EP015071OEI
CSB-BP015071OEI
CSB-MP015071OEI
CSB-EP015071OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Thymidylate kinase (TMK) CSB-YP007226VAA
CSB-EP007226VAA
CSB-BP007226VAA
CSB-MP007226VAA
CSB-EP007226VAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribosome-recycling factor (frr) CSB-YP014941SUL
CSB-EP014941SUL
CSB-BP014941SUL
CSB-MP014941SUL
CSB-EP014941SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Hemoglobin subunit beta (HBB) CSB-YP010150EQU
CSB-EP010150EQU
CSB-BP010150EQU
CSB-MP010150EQU
CSB-EP010150EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cygnus olor Hemoglobin subunit beta (HBB) CSB-YP010150CVK
CSB-EP010150CVK
CSB-BP010150CVK
CSB-MP010150CVK
CSB-EP010150CVK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana plumbaginifolia Eukaryotic translation initiation factor 5A-1 (EIF-5A1) CSB-YP007573NHC
CSB-EP007573NHC
CSB-BP007573NHC
CSB-MP007573NHC
CSB-EP007573NHC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Cu-Zn] (sodC) CSB-YP022397LNY
CSB-EP022397LNY
CSB-BP022397LNY
CSB-MP022397LNY
CSB-EP022397LNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trematomus bernacchii L-lactate dehydrogenase A chain (ldha) CSB-YP012832TPM
CSB-EP012832TPM
CSB-BP012832TPM
CSB-MP012832TPM
CSB-EP012832TPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H3.2 CSB-YP010419TBI
CSB-EP010419TBI
CSB-BP010419TBI
CSB-MP010419TBI
CSB-EP010419TBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Histone H3.2 CSB-YP010419TBQ
CSB-EP010419TBQ
CSB-BP010419TBQ
CSB-MP010419TBQ
CSB-EP010419TBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pangasianodon gigas Somatotropin (gh) CSB-YP009407PAX
CSB-EP009407PAX
CSB-BP009407PAX
CSB-MP009407PAX
CSB-EP009407PAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Thymidine kinase (TK) CSB-YP023577EFL
CSB-EP023577EFL
CSB-BP023577EFL
CSB-MP023577EFL
CSB-EP023577EFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cichlasoma centrarchus Cytochrome b (mt-cyb), partial CSB-YP015075CKL1
CSB-EP015075CKL1
CSB-BP015075CKL1
CSB-MP015075CKL1
CSB-EP015075CKL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma lanceolatum NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078BVZ1
CSB-EP015078BVZ1
CSB-BP015078BVZ1
CSB-MP015078BVZ1
CSB-EP015078BVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Histone H3.2 (H3C2) CSB-YP010419ZAX
CSB-EP010419ZAX
CSB-BP010419ZAX
CSB-MP010419ZAX
CSB-EP010419ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus nerka NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080OBI1
CSB-EP015080OBI1
CSB-BP015080OBI1
CSB-MP015080OBI1
CSB-EP015080OBI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Hemoglobin subunit alpha (HBA1) CSB-YP010147EQU
CSB-EP010147EQU
CSB-BP010147EQU
CSB-MP010147EQU
CSB-EP010147EQU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucoraja erinacea Gap junction delta-2 protein, partial CSB-YP009461LFI1
CSB-EP009461LFI1
CSB-BP009461LFI1
CSB-MP009461LFI1
CSB-EP009461LFI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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