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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Galleria mellonella Defensin CSB-YP006652GET
CSB-EP006652GET
CSB-BP006652GET
CSB-MP006652GET
CSB-EP006652GET-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Merluccius senegalensis Nucleoside diphosphate kinase B (nme2) CSB-YP015886MEL
CSB-EP015886MEL
CSB-BP015886MEL
CSB-MP015886MEL
CSB-EP015886MEL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Merluccius hubbsi Nucleoside diphosphate kinase B (nme2) CSB-YP015886MEG
CSB-EP015886MEG
CSB-BP015886MEG
CSB-MP015886MEG
CSB-EP015886MEG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Merluccius australis australis Nucleoside diphosphate kinase B (nme2) CSB-YP015886MEB
CSB-EP015886MEB
CSB-BP015886MEB
CSB-MP015886MEB
CSB-EP015886MEB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macruronus magellanicus Nucleoside diphosphate kinase B (nme2) CSB-YP015886MAR
CSB-EP015886MAR
CSB-BP015886MAR
CSB-MP015886MAR
CSB-EP015886MAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catharacta maccormicki Hemoglobin subunit alpha-2 CSB-YP010148CER
CSB-EP010148CER
CSB-BP010148CER
CSB-MP010148CER
CSB-EP010148CER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spinacia oleracea 60S ribosomal protein L13a (RPL13A) CSB-YP020130FKI
CSB-EP020130FKI
CSB-BP020130FKI
CSB-MP020130FKI
CSB-EP020130FKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus F-actin-capping protein subunit beta (CAPZB) CSB-YP004514MRG
CSB-EP004514MRG
CSB-BP004514MRG
CSB-MP004514MRG
CSB-EP004514MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Fumarylacetoacetate hydrolase domain-containing protein 2A (FAHD2) CSB-YP007967MRG
CSB-EP007967MRG
CSB-BP007967MRG
CSB-MP007967MRG
CSB-EP007967MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Mitochondrial inner membrane protein (IMMT) CSB-YP011690MRG
CSB-EP011690MRG
CSB-BP011690MRG
CSB-MP011690MRG
CSB-EP011690MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emys orbicularis Beta-defensin 1 CSB-YP006662EBQ
CSB-EP006662EBQ
CSB-BP006662EBQ
CSB-MP006662EBQ
CSB-EP006662EBQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lama guanicoe Osteocalcin (BGLAP) CSB-YP002682LBT
CSB-EP002682LBT
CSB-BP002682LBT
CSB-MP002682LBT
CSB-EP002682LBT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein odd-skipped-related 1 (osr1) CSB-YP017262XBE
CSB-EP017262XBE
CSB-BP017262XBE
CSB-MP017262XBE
CSB-EP017262XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Siganus canaliculatus L-amino-acid oxidase CSB-YP011660SGJ
CSB-EP011660SGJ
CSB-BP011660SGJ
CSB-MP011660SGJ
CSB-EP011660SGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription initiation factor TFIID subunit 2 (taf2), partial CSB-YP023088SXV
CSB-EP023088SXV
CSB-BP023088SXV
CSB-MP023088SXV
CSB-EP023088SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe U6 snRNA-associated Sm-like protein LSm1 (lsm1) CSB-YP013199SXV
CSB-EP013199SXV
CSB-BP013199SXV
CSB-MP013199SXV
CSB-EP013199SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mediator of RNA polymerase II transcription subunit 10 (med10) CSB-YP013641SXV
CSB-EP013641SXV
CSB-BP013641SXV
CSB-MP013641SXV
CSB-EP013641SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eleutherodactylus coqui Homeobox protein DLX-2 (DLX2) CSB-YP006953EBJ
CSB-EP006953EBJ
CSB-BP006953EBJ
CSB-MP006953EBJ
CSB-EP006953EBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Atractaspis micropholis Cytochrome b (MT-CYB), partial CSB-YP015075AQP1
CSB-EP015075AQP1
CSB-BP015075AQP1
CSB-MP015075AQP1
CSB-EP015075AQP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 6-phosphofructokinase (pfkA), partial CSB-YP017822DKK
CSB-EP017822DKK
CSB-BP017822DKK
CSB-MP017822DKK
CSB-EP017822DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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