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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Splicing factor U2AF 65 kDa subunit (uaf-1) CSB-YP025408CXX
CSB-EP025408CXX
CSB-BP025408CXX
CSB-MP025408CXX
CSB-EP025408CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase delta catalytic subunit (F10C2.4), partial CSB-YP018303CXY
CSB-EP018303CXY
CSB-BP018303CXY
CSB-MP018303CXY
CSB-EP018303CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein max (Max) CSB-YP013527DLU
CSB-EP013527DLU
CSB-BP013527DLU
CSB-MP013527DLU
CSB-EP013527DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes togoi Triosephosphate isomerase (Tpi) CSB-YP024102ADH
CSB-EP024102ADH
CSB-BP024102ADH
CSB-MP024102ADH
CSB-EP024102ADH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 14-3-3 protein epsilon (14-3-3epsilon) CSB-YP026287DLU
CSB-EP026287DLU
CSB-BP026287DLU
CSB-MP026287DLU
CSB-EP026287DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Azemiops feae NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079ATT1
CSB-EP015079ATT1
CSB-BP015079ATT1
CSB-MP015079ATT1
CSB-EP015079ATT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome c oxidase subunit 3 (COX3), partial CSB-YP015074DOA1
CSB-EP015074DOA1
CSB-BP015074DOA1
CSB-MP015074DOA1
CSB-EP015074DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076DOA
CSB-EP015076DOA
CSB-BP015076DOA
CSB-MP015076DOA
CSB-EP015076DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Calocitta formosa Cytochrome b (MT-CYB), partial CSB-YP015075CBH
CSB-EP015075CBH
CSB-BP015075CBH
CSB-MP015075CBH
CSB-EP015075CBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mammuthus primigenius Cytochrome b (MT-CYB), partial CSB-YP015075MBW
CSB-EP015075MBW
CSB-BP015075MBW
CSB-MP015075MBW
CSB-EP015075MBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus robustus NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080MAK1
CSB-EP015080MAK1
CSB-BP015080MAK1
CSB-MP015080MAK1
CSB-EP015080MAK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo abelii NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077PYX
CSB-EP015077PYX
CSB-BP015077PYX
CSB-MP015077PYX
CSB-EP015077PYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perameles nasuta Cytochrome b (MT-CYB), partial CSB-YP015075PFP
CSB-EP015075PFP
CSB-BP015075PFP
CSB-MP015075PFP
CSB-EP015075PFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protobothrops elegans NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079PSR1
CSB-EP015079PSR1
CSB-BP015079PSR1
CSB-MP015079PSR1
CSB-EP015079PSR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothrops atrox Cytochrome b (MT-CYB), partial CSB-YP015075BUU1
CSB-EP015075BUU1
CSB-BP015075BUU1
CSB-MP015075BUU1
CSB-EP015075BUU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum melongena 60S ribosomal protein L10 (RPL10) CSB-YP020106SIQ
CSB-EP020106SIQ
CSB-BP020106SIQ
CSB-MP020106SIQ
CSB-EP020106SIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl carrier protein (acpP) CSB-YP015636DPS
CSB-EP015636DPS
CSB-BP015636DPS
CSB-MP015636DPS
CSB-EP015636DPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fremyella diplosiphon Peptide deformylase (def) CSB-YP017707FCY
CSB-EP017707FCY
CSB-BP017707FCY
CSB-MP017707FCY
CSB-EP017707FCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Geranylgeranyl pyrophosphate synthase (gds) CSB-YP009393FPM
CSB-EP009393FPM
CSB-BP009393FPM
CSB-MP009393FPM
CSB-EP009393FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Peptide chain release factor 1 (prfA) CSB-YP015197TNT
CSB-EP015197TNT
CSB-BP015197TNT
CSB-MP015197TNT
CSB-EP015197TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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