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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Dictyostelium discoideum 60S ribosomal protein L8 (rpl8) CSB-YP020323DKK
CSB-EP020323DKK
CSB-BP020323DKK
CSB-MP020323DKK
CSB-EP020323DKK-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (THYA) CSB-YP025393EXG
CSB-EP025393EXG
CSB-BP025393EXG
CSB-MP025393EXG
CSB-EP025393EXG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides fragilis ATP synthase subunit beta (atpD), partial CSB-YP002350BDQ
CSB-EP002350BDQ
CSB-BP002350BDQ
CSB-MP002350BDQ
CSB-EP002350BDQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oreochromis niloticus Somatotropin (gh) CSB-YP009407OET
CSB-EP009407OET
CSB-BP009407OET
CSB-MP009407OET
CSB-EP009407OET-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleoside diphosphate-linked moiety X motif 6 (nudt6) CSB-YP016176XBE
CSB-EP016176XBE
CSB-BP016176XBE
CSB-MP016176XBE
CSB-EP016176XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Dynactin subunit 1 (Gl), partial CSB-YP006564DLU
CSB-EP006564DLU
CSB-BP006564DLU
CSB-MP006564DLU
CSB-EP006564DLU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Glutamine synthetase (glnA) CSB-YP009553RLC
CSB-EP009553RLC
CSB-BP009553RLC
CSB-MP009553RLC
CSB-EP009553RLC-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae F-actin-capping protein subunit beta (CAP2) CSB-YP004514SVG
CSB-EP004514SVG
CSB-BP004514SVG
CSB-MP004514SVG
CSB-EP004514SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima Elongation factor Tu (tuf) CSB-YP025342TNJ
CSB-EP025342TNJ
CSB-BP025342TNJ
CSB-MP025342TNJ
CSB-EP025342TNJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Myosin-6 (MYH6), partial CSB-YP015299MRG
CSB-EP015299MRG
CSB-BP015299MRG
CSB-MP015299MRG
CSB-EP015299MRG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vigna radiata var. radiata V-type proton ATPase catalytic subunit A, partial CSB-YP002396VFL
CSB-EP002396VFL
CSB-BP002396VFL
CSB-MP002396VFL
CSB-EP002396VFL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tarsius syrichta Hemoglobin subunit delta (HBD) CSB-YP010152TLJ
CSB-EP010152TLJ
CSB-BP010152TLJ
CSB-MP010152TLJ
CSB-EP010152TLJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Nuclear factor 1 X-type (NFIX) CSB-YP015758MRG
CSB-EP015758MRG
CSB-BP015758MRG
CSB-MP015758MRG
CSB-EP015758MRG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c1 (petC), partial CSB-YP006327PBZ1
CSB-EP006327PBZ1
CSB-BP006327PBZ1
CSB-MP006327PBZ1
CSB-EP006327PBZ1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium/potassium-transporting ATPase subunit beta-2 (Atp1b2), partial CSB-YP002327RA1
CSB-EP002327RA1
CSB-BP002327RA1
CSB-MP002327RA1
CSB-EP002327RA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Platelet-derived growth factor subunit A (pdgfa) CSB-YP017708XBE
CSB-EP017708XBE
CSB-BP017708XBE
CSB-MP017708XBE
CSB-EP017708XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas platyrhynchos L-lactate dehydrogenase B chain (LDHB) CSB-YP012841BZD
CSB-EP012841BZD
CSB-BP012841BZD
CSB-MP012841BZD
CSB-EP012841BZD-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Ornithine decarboxylase (ODC1) CSB-YP016269DXU
CSB-EP016269DXU
CSB-BP016269DXU
CSB-MP016269DXU
CSB-EP016269DXU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Cytochrome c oxidase subunit 3 (COIII), partial CSB-YP015074SXR1
CSB-EP015074SXR1
CSB-BP015074SXR1
CSB-MP015074SXR1
CSB-EP015074SXR1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Exportin-1 (xpo1), partial CSB-YP026221SXV
CSB-EP026221SXV
CSB-BP026221SXV
CSB-MP026221SXV
CSB-EP026221SXV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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