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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Solanum lycopersicum Histone H4 CSB-YP010429SZY
CSB-EP010429SZY
CSB-BP010429SZY
CSB-MP010429SZY
CSB-EP010429SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Cytochrome b (MT-CYB), partial CSB-YP015075DXJ
CSB-EP015075DXJ
CSB-BP015075DXJ
CSB-MP015075DXJ
CSB-EP015075DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tachyglossus aculeatus aculeatus Sperm protamine P1 (PRM1) CSB-YP018728TKU
CSB-EP018728TKU
CSB-BP018728TKU
CSB-MP018728TKU
CSB-EP018728TKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Metabotropic glutamate receptor 6 (Grm6), partial CSB-YP009936RA
CSB-EP009936RA
CSB-BP009936RA
CSB-MP009936RA
CSB-EP009936RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Didelphis marsupialis virginiana Alpha-2C adrenergic receptor (ADRA2C), partial CSB-YP001390DKL1
CSB-EP001390DKL1
CSB-BP001390DKL1
CSB-MP001390DKL1
CSB-EP001390DKL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP017716ABI
CSB-EP017716ABI
CSB-BP017716ABI
CSB-MP017716ABI
CSB-EP017716ABI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scyliorhinus canicula Urotensin-2 CSB-YP025771SYA
CSB-EP025771SYA
CSB-BP025771SYA
CSB-MP025771SYA
CSB-EP025771SYA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant AP-2 complex subunit mu (dpy-23) CSB-YP001872CXY
CSB-EP001872CXY
CSB-BP001872CXY
CSB-MP001872CXY
CSB-EP001872CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Neurofilament light polypeptide (nefl), partial CSB-YP015688XBE
CSB-EP015688XBE
CSB-BP015688XBE
CSB-MP015688XBE
CSB-EP015688XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marchantia polymorpha Succinate dehydrogenase cytochrome b560 subunit (SDH3), partial CSB-YP020908MCB1
CSB-EP020908MCB1
CSB-BP020908MCB1
CSB-MP020908MCB1
CSB-EP020908MCB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acylphosphatase-2 (Acyp2) CSB-YP001266RA
CSB-EP001266RA
CSB-BP001266RA
CSB-MP001266RA
CSB-EP001266RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amidophosphoribosyltransferase (purF) CSB-YP018427LMH
CSB-EP018427LMH
CSB-BP018427LMH
CSB-MP018427LMH
CSB-EP018427LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Glycogenin-1 (GLG1), partial CSB-YP010070SVG
CSB-EP010070SVG
CSB-BP010070SVG
CSB-MP010070SVG
CSB-EP010070SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 60S ribosomal protein L19 (RpL19) CSB-YP020160DLU
CSB-EP020160DLU
CSB-BP020160DLU
CSB-MP020160DLU
CSB-EP020160DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Dimethylaniline monooxygenase [N-oxide-forming] 1 (Fmo1) CSB-YP008746RA
CSB-EP008746RA
CSB-BP008746RA
CSB-MP008746RA
CSB-EP008746RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Fibroblast growth factor 3 (fgf3) CSB-YP008630XBE
CSB-EP008630XBE
CSB-BP008630XBE
CSB-MP008630XBE
CSB-EP008630XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ursus arctos Sex-determining region Y protein (SRY) CSB-YP022699UBO
CSB-EP022699UBO
CSB-BP022699UBO
CSB-MP022699UBO
CSB-EP022699UBO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neuronal calcium sensor 1 (ncs-1) CSB-YP008983CXY
CSB-EP008983CXY
CSB-BP008983CXY
CSB-MP008983CXY
CSB-EP008983CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB9 (RpII15) CSB-YP018336DLU
CSB-EP018336DLU
CSB-BP018336DLU
CSB-MP018336DLU
CSB-EP018336DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Legionella pneumophila subsp. pneumophila Aconitate hydratase (acn), partial CSB-YP001162LDL
CSB-EP001162LDL
CSB-BP001162LDL
CSB-MP001162LDL
CSB-EP001162LDL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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