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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus influenzae Lactoylglutathione lyase (gloA) CSB-YP009511HTA
CSB-EP009511HTA
CSB-BP009511HTA
CSB-MP009511HTA
CSB-EP009511HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae ADP-ribose pyrophosphatase (nudF) CSB-YP016179HTA
CSB-EP016179HTA
CSB-BP016179HTA
CSB-MP016179HTA
CSB-EP016179HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Peptide deformylase (def) CSB-YP017707HTA
CSB-EP017707HTA
CSB-BP017707HTA
CSB-MP017707HTA
CSB-EP017707HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae UTP--glucose-1-phosphate uridylyltransferase (galU) CSB-YP025569HTA
CSB-EP025569HTA
CSB-BP025569HTA
CSB-MP025569HTA
CSB-EP025569HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Transaldolase (tal) CSB-YP023112HTA
CSB-EP023112HTA
CSB-BP023112HTA
CSB-MP023112HTA
CSB-EP023112HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917HTA
CSB-EP022917HTA
CSB-BP022917HTA
CSB-MP022917HTA
CSB-EP022917HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Aminopeptidase N (pepN), partial CSB-YP001827HTA
CSB-EP001827HTA
CSB-BP001827HTA
CSB-MP001827HTA
CSB-EP001827HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae tRNA pseudouridine synthase A (truA) CSB-YP019086HTA
CSB-EP019086HTA
CSB-BP019086HTA
CSB-MP019086HTA
CSB-EP019086HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Molybdopterin synthase catalytic subunit (moaE) CSB-YP014706HTA
CSB-EP014706HTA
CSB-BP014706HTA
CSB-MP014706HTA
CSB-EP014706HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Oligoribonuclease (orn) CSB-YP019586HTA
CSB-EP019586HTA
CSB-BP019586HTA
CSB-MP019586HTA
CSB-EP019586HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-CoA acetyltransferase (thi) CSB-YP001134DUN
CSB-EP001134DUN
CSB-BP001134DUN
CSB-MP001134DUN
CSB-EP001134DUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP017878FFT1
CSB-EP017878FFT1
CSB-BP017878FFT1
CSB-MP017878FFT1
CSB-EP017878FFT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis U1 small nuclear ribonucleoprotein A (snrpa) CSB-YP022327XBE
CSB-EP022327XBE
CSB-BP022327XBE
CSB-MP022327XBE
CSB-EP022327XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Translocon-associated protein subunit alpha (ssr1), partial CSB-YP022717OEI1
CSB-EP022717OEI1
CSB-BP022717OEI1
CSB-MP022717OEI1
CSB-EP022717OEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Translocon-associated protein subunit alpha (At2g21160), partial CSB-YP022717DOA1
CSB-EP022717DOA1
CSB-BP022717DOA1
CSB-MP022717DOA1
CSB-EP022717DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Coatomer subunit beta (betaCop), partial CSB-YP005783DLU
CSB-EP005783DLU
CSB-BP005783DLU
CSB-MP005783DLU
CSB-EP005783DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Procambarus clarkii Actin CSB-YP001205PRX
CSB-EP001205PRX
CSB-BP001205PRX
CSB-MP001205PRX
CSB-EP001205PRX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 60S ribosomal protein L10 (RPL10) CSB-YP020106ZAX
CSB-EP020106ZAX
CSB-BP020106ZAX
CSB-MP020106ZAX
CSB-EP020106ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Lutropin subunit beta (LHB) CSB-YP012910MQV
CSB-EP012910MQV
CSB-BP012910MQV
CSB-MP012910MQV
CSB-EP012910MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Progonadoliberin-1 (gnrh1) CSB-YP009635XBE
CSB-EP009635XBE
CSB-BP009635XBE
CSB-MP009635XBE
CSB-EP009635XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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