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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster Synaptosomal-associated protein 25 (Snap25) CSB-YP021873DLU
CSB-EP021873DLU
CSB-BP021873DLU
CSB-MP021873DLU
CSB-EP021873DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Cysteine desulfurase (nifS) CSB-YP015769BVW
CSB-EP015769BVW
CSB-BP015769BVW
CSB-MP015769BVW
CSB-EP015769BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Balaenoptera acutorostrata Glycoprotein hormones alpha chain (CGA) CSB-YP005293BDY
CSB-EP005293BDY
CSB-BP005293BDY
CSB-MP005293BDY
CSB-EP005293BDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Cytochrome b-c1 complex subunit 10 (QCR10), partial CSB-YP025663SVG1
CSB-EP025663SVG1
CSB-BP025663SVG1
CSB-MP025663SVG1
CSB-EP025663SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrrhocoris apterus Defensin CSB-YP006652PXV
CSB-EP006652PXV
CSB-BP006652PXV
CSB-MP006652PXV
CSB-EP006652PXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus albus Tubulin beta-1 chain (TUBB1) CSB-YP025319LJQ
CSB-EP025319LJQ
CSB-BP025319LJQ
CSB-MP025319LJQ
CSB-EP025319LJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Methionine synthase (metH), partial CSB-YP015196SXB
CSB-EP015196SXB
CSB-BP015196SXB
CSB-MP015196SXB
CSB-EP015196SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Camelus dromedarius Lysozyme C (LYZ) CSB-YP013283CYV
CSB-EP013283CYV
CSB-BP013283CYV
CSB-MP013283CYV
CSB-EP013283CYV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Calponin-1 (CNN1) CSB-YP005655MQV
CSB-EP005655MQV
CSB-BP005655MQV
CSB-MP005655MQV
CSB-EP005655MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ATP synthase subunit b (atpF), partial CSB-YP002358BRJ1
CSB-EP002358BRJ1
CSB-BP002358BRJ1
CSB-MP002358BRJ1
CSB-EP002358BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Cyclin-A2 (CCNA2) CSB-YP004805MRG
CSB-EP004805MRG
CSB-BP004805MRG
CSB-MP004805MRG
CSB-EP004805MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lama guanicoe pacos Somatotropin (GH1) CSB-YP009407LBU
CSB-EP009407LBU
CSB-BP009407LBU
CSB-MP009407LBU
CSB-EP009407LBU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (bfmBB) CSB-YP006530BRJ
CSB-EP006530BRJ
CSB-BP006530BRJ
CSB-MP006530BRJ
CSB-EP006530BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Cytochrome c (cycA) CSB-YP006328EKF
CSB-EP006328EKF
CSB-BP006328EKF
CSB-MP006328EKF
CSB-EP006328EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA replication licensing factor MCM7 (MCM7), partial CSB-YP013597SVG
CSB-EP013597SVG
CSB-BP013597SVG
CSB-MP013597SVG
CSB-EP013597SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Tricarboxylate transport protein (CTP1), partial CSB-YP021475SVG1
CSB-EP021475SVG1
CSB-BP021475SVG1
CSB-MP021475SVG1
CSB-EP021475SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae U6 snRNA-associated Sm-like protein LSm2 (LSM2) CSB-YP013205SVG
CSB-EP013205SVG
CSB-BP013205SVG
CSB-MP013205SVG
CSB-EP013205SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Structure-specific endonuclease subunit SLX1 (SLX1) CSB-YP009442SVG
CSB-EP009442SVG
CSB-BP009442SVG
CSB-MP009442SVG
CSB-EP009442SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Protein transport protein Sec61 subunit gamma (Os02g0178400, LOC_Os02g08180), partial CSB-YP020959OFG1
CSB-EP020959OFG1
CSB-BP020959OFG1
CSB-MP020959OFG1
CSB-EP020959OFG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB7 (RPB19) CSB-YP018334DOA
CSB-EP018334DOA
CSB-BP018334DOA
CSB-MP018334DOA
CSB-EP018334DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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