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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Haemophilus influenzae Amidophosphoribosyltransferase (purF), partial CSB-YP018427HTA
CSB-EP018427HTA
CSB-BP018427HTA
CSB-MP018427HTA
CSB-EP018427HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Elongation factor Ts (tsf) CSB-YP025124TNT
CSB-EP025124TNT
CSB-BP025124TNT
CSB-MP025124TNT
CSB-EP025124TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Quinone oxidoreductase (qor) CSB-YP006028EZX
CSB-EP006028EZX
CSB-BP006028EZX
CSB-MP006028EZX
CSB-EP006028EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Elongation factor Tu (tufA) CSB-YP025342HTA
CSB-EP025342HTA
CSB-BP025342HTA
CSB-MP025342HTA
CSB-EP025342HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Peptidyl-prolyl cis-trans isomerase D (ppiD), partial CSB-YP018474HTA1
CSB-EP018474HTA1
CSB-BP018474HTA1
CSB-MP018474HTA1
CSB-EP018474HTA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Argininosuccinate synthase (argG) CSB-YP002234HTA
CSB-EP002234HTA
CSB-BP002234HTA
CSB-MP002234HTA
CSB-EP002234HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Ornithine decarboxylase (speF), partial CSB-YP016269HTA
CSB-EP016269HTA
CSB-BP016269HTA
CSB-MP016269HTA
CSB-EP016269HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae DNA-3-methyladenine glycosylase (tag) CSB-YP014749HTA
CSB-EP014749HTA
CSB-BP014749HTA
CSB-MP014749HTA
CSB-EP014749HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Translation initiation factor IF-2 (infB), partial CSB-YP015164HTA
CSB-EP015164HTA
CSB-BP015164HTA
CSB-MP015164HTA
CSB-EP015164HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Phosphoserine aminotransferase (serC) CSB-YP018838HTA
CSB-EP018838HTA
CSB-BP018838HTA
CSB-MP018838HTA
CSB-EP018838HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Xylulose kinase (xylB) CSB-YP026240HTA
CSB-EP026240HTA
CSB-BP026240HTA
CSB-MP026240HTA
CSB-EP026240HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Single-stranded DNA-binding protein (ssb) CSB-YP022706HTA
CSB-EP022706HTA
CSB-BP022706HTA
CSB-MP022706HTA
CSB-EP022706HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Thymidylate synthase (thyA) CSB-YP025393HTA
CSB-EP025393HTA
CSB-BP025393HTA
CSB-MP025393HTA
CSB-EP025393HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Aspartate aminotransferase (aspC) CSB-YP009681HTA
CSB-EP009681HTA
CSB-BP009681HTA
CSB-MP009681HTA
CSB-EP009681HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Malate dehydrogenase (mdh) CSB-YP013623HTA
CSB-EP013623HTA
CSB-BP013623HTA
CSB-MP013623HTA
CSB-EP013623HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Ribonuclease 3 (rnc) CSB-YP019809HTA
CSB-EP019809HTA
CSB-BP019809HTA
CSB-MP019809HTA
CSB-EP019809HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Lipoyl synthase (lipA) CSB-YP012927HTA
CSB-EP012927HTA
CSB-BP012927HTA
CSB-MP012927HTA
CSB-EP012927HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Inorganic pyrophosphatase (ppa) CSB-YP018410HTA
CSB-EP018410HTA
CSB-BP018410HTA
CSB-MP018410HTA
CSB-EP018410HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae N-acetylneuraminate lyase (nanA) CSB-YP015994HTA
CSB-EP015994HTA
CSB-BP015994HTA
CSB-MP015994HTA
CSB-EP015994HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415HTA
CSB-EP015415HTA
CSB-BP015415HTA
CSB-MP015415HTA
CSB-EP015415HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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