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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae RNA polymerase-associated protein LEO1 (LEO1) CSB-YP012869SVG
CSB-EP012869SVG
CSB-BP012869SVG
CSB-MP012869SVG
CSB-EP012869SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima Elongation factor G (fusA), partial CSB-YP009373TNJ
CSB-EP009373TNJ
CSB-BP009373TNJ
CSB-MP009373TNJ
CSB-EP009373TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Hyaluronidase PH-20 (SPAM1) CSB-YP022471MOV
CSB-EP022471MOV
CSB-BP022471MOV
CSB-MP022471MOV
CSB-EP022471MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus Cytochrome b (MT-CYB), partial CSB-YP015075HAU
CSB-EP015075HAU
CSB-BP015075HAU
CSB-MP015075HAU
CSB-EP015075HAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076HAU
CSB-EP015076HAU
CSB-BP015076HAU
CSB-MP015076HAU
CSB-EP015076HAU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halichoerus grypus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078HAU1
CSB-EP015078HAU1
CSB-BP015078HAU1
CSB-MP015078HAU1
CSB-EP015078HAU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribose-phosphate pyrophosphokinase 2 (PRS2) CSB-YP018779SVG
CSB-EP018779SVG
CSB-BP018779SVG
CSB-MP018779SVG
CSB-EP018779SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GMP synthase [glutamine-hydrolyzing] (GUA1), partial CSB-YP009582SVG
CSB-EP009582SVG
CSB-BP009582SVG
CSB-MP009582SVG
CSB-EP009582SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphoacetylglucosamine mutase (PCM1), partial CSB-YP017869SVG
CSB-EP017869SVG
CSB-BP017869SVG
CSB-MP017869SVG
CSB-EP017869SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Phosphoglucomutase-1 (Pgm1) CSB-YP017866RA
CSB-EP017866RA
CSB-BP017866RA
CSB-MP017866RA
CSB-EP017866RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Protein disulfide-isomerase A6 (PDIA6) CSB-YP017724MRG
CSB-EP017724MRG
CSB-BP017724MRG
CSB-MP017724MRG
CSB-EP017724MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Inositol monophosphatase 1 (INM1) CSB-YP011694SVG
CSB-EP011694SVG
CSB-BP011694SVG
CSB-MP011694SVG
CSB-EP011694SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 29 (VPS29) CSB-YP025901SVG
CSB-EP025901SVG
CSB-BP025901SVG
CSB-MP025901SVG
CSB-EP025901SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin-like modifier-activating enzyme ATG7 (ATG7), partial CSB-YP002294SVG
CSB-EP002294SVG
CSB-BP002294SVG
CSB-MP002294SVG
CSB-EP002294SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein sorting-associated protein 45 (VPS45), partial CSB-YP025912SVG
CSB-EP025912SVG
CSB-BP025912SVG
CSB-MP025912SVG
CSB-EP025912SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ribosomal RNA-processing protein 8 (RRP8) CSB-YP020531SVG
CSB-EP020531SVG
CSB-BP020531SVG
CSB-MP020531SVG
CSB-EP020531SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus 40S ribosomal protein SA (Rpsa) CSB-YP020485DXU
CSB-EP020485DXU
CSB-BP020485DXU
CSB-MP020485DXU
CSB-EP020485DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Long-chain-fatty-acid--CoA ligase 3 (FAA3), partial CSB-YP001192SVG
CSB-EP001192SVG
CSB-BP001192SVG
CSB-MP001192SVG
CSB-EP001192SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S acidic ribosomal protein P0 (LCP0) CSB-YP020336LOD
CSB-EP020336LOD
CSB-BP020336LOD
CSB-MP020336LOD
CSB-EP020336LOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae V-type proton ATPase subunit F (VMA7) CSB-YP002407SVG
CSB-EP002407SVG
CSB-BP002407SVG
CSB-MP002407SVG
CSB-EP002407SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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