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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Clostridium acetobutylicum Electron transfer flavoprotein subunit beta (etfB) CSB-YP007845DUB
CSB-EP007845DUB
CSB-BP007845DUB
CSB-MP007845DUB
CSB-EP007845DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein max (max) CSB-YP013527DIL
CSB-EP013527DIL
CSB-BP013527DIL
CSB-MP013527DIL
CSB-EP013527DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioredoxin reductase (trxB) CSB-YP025388MVZ
CSB-EP025388MVZ
CSB-BP025388MVZ
CSB-MP025388MVZ
CSB-EP025388MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Protein disulfide-isomerase (PDI) CSB-YP017342TQN
CSB-EP017342TQN
CSB-BP017342TQN
CSB-MP017342TQN
CSB-EP017342TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina 40S ribosomal protein S9 (RPS9) CSB-YP020482EXI
CSB-EP020482EXI
CSB-BP020482EXI
CSB-MP020482EXI
CSB-EP020482EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S25 (rps-25) CSB-YP020406CXY
CSB-EP020406CXY
CSB-BP020406CXY
CSB-MP020406CXY
CSB-EP020406CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua 60S ribosomal protein L22 (rpl22) CSB-YP020182GER
CSB-EP020182GER
CSB-BP020182GER
CSB-MP020182GER
CSB-EP020182GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spodoptera frugiperda TATA-box-binding protein (Tbp) CSB-YP023239FKQ
CSB-EP023239FKQ
CSB-BP023239FKQ
CSB-MP023239FKQ
CSB-EP023239FKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycerol-3-phosphate dehydrogenase (glpD), partial CSB-YP009711MVN
CSB-EP009711MVN
CSB-BP009711MVN
CSB-MP009711MVN
CSB-EP009711MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanidioschyzon merolae Actin CSB-YP001205DZU
CSB-EP001205DZU
CSB-BP001205DZU
CSB-MP001205DZU
CSB-EP001205DZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Geranylgeranyl transferase type-1 subunit beta (PGGT1B) CSB-YP017855HU
CSB-EP017855HU
CSB-BP017855HU
CSB-MP017855HU
CSB-EP017855HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MLA
CSB-EP022398MLA
CSB-BP022398MLA
CSB-MP022398MLA
CSB-EP022398MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Superoxide dismutase [Mn] (sodA) CSB-YP022398EZX
CSB-EP022398EZX
CSB-BP022398EZX
CSB-MP022398EZX
CSB-EP022398EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa V-type proton ATPase subunit d (vma-6), partial CSB-YP002401NHA
CSB-EP002401NHA
CSB-BP002401NHA
CSB-MP002401NHA
CSB-EP002401NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaffia rhodozyma Actin CSB-YP001205EUI
CSB-EP001205EUI
CSB-BP001205EUI
CSB-MP001205EUI
CSB-EP001205EUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio cynocephalus Integrin alpha-IIb (ITGA2B), partial CSB-YP011865PBR
CSB-EP011865PBR
CSB-BP011865PBR
CSB-MP011865PBR
CSB-EP011865PBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium/glucose cotransporter 2 (Slc5a2), partial CSB-YP021679RA
CSB-EP021679RA
CSB-BP021679RA
CSB-MP021679RA
CSB-EP021679RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 10 (UBP10), partial CSB-YP025696SVG
CSB-EP025696SVG
CSB-BP025696SVG
CSB-MP025696SVG
CSB-EP025696SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Anaphase-promoting complex subunit 1 (APC1), partial CSB-YP001694SVG
CSB-EP001694SVG
CSB-BP001694SVG
CSB-MP001694SVG
CSB-EP001694SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) CSB-YP015412SVG
CSB-EP015412SVG
CSB-BP015412SVG
CSB-MP015412SVG
CSB-EP015412SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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