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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Choristoneura biennis Cytochrome c oxidase subunit 1 (COI), partial CSB-YP015072CJS
CSB-EP015072CJS
CSB-BP015072CJS
CSB-MP015072CJS
CSB-EP015072CJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Damaliscus pygargus phillipsi Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073DAG1
CSB-EP015073DAG1
CSB-BP015073DAG1
CSB-MP015073DAG1
CSB-EP015073DAG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Lanosterol 14-alpha demethylase (ERG11), partial CSB-YP006459CZI
CSB-EP006459CZI
CSB-BP006459CZI
CSB-MP006459CZI
CSB-EP006459CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracentrotus lividus 40S ribosomal protein S15a (RPS15A) CSB-YP020373ERM
CSB-EP020373ERM
CSB-BP020373ERM
CSB-MP020373ERM
CSB-EP020373ERM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe T-complex protein 1 subunit delta (cct4), partial CSB-YP004859SXV
CSB-EP004859SXV
CSB-BP004859SXV
CSB-MP004859SXV
CSB-EP004859SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Ribulose-phosphate 3-epimerase (cbbE) CSB-YP020097RLC
CSB-EP020097RLC
CSB-BP020097RLC
CSB-MP020097RLC
CSB-EP020097RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Amiloride-sensitive sodium channel subunit alpha (scnn1a), partial CSB-YP020848XBE
CSB-EP020848XBE
CSB-BP020848XBE
CSB-MP020848XBE
CSB-EP020848XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 60S ribosomal protein L32 (RPL32) CSB-YP020237ZAX
CSB-EP020237ZAX
CSB-BP020237ZAX
CSB-MP020237ZAX
CSB-EP020237ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leontopithecus rosalia Hemoglobin subunit epsilon (HBE1) CSB-YP010153LLO
CSB-EP010153LLO
CSB-BP010153LLO
CSB-MP010153LLO
CSB-EP010153LLO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Arrestin-C (arr3) CSB-YP002133XBE
CSB-EP002133XBE
CSB-BP002133XBE
CSB-MP002133XBE
CSB-EP002133XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Solute carrier family 15 member 1 (Slc15a1), partial CSB-YP021398RA
CSB-EP021398RA
CSB-BP021398RA
CSB-MP021398RA
CSB-EP021398RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Cytochrome P450 2E1 (CYP2E1) CSB-YP006425MRG
CSB-EP006425MRG
CSB-BP006425MRG
CSB-MP006425MRG
CSB-EP006425MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human N-acylglucosamine 2-epimerase (RENBP) CSB-YP019562HU
CSB-EP019562HU
CSB-BP019562HU
CSB-MP019562HU
CSB-EP019562HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Tubulin beta-1 chain (TUBB1) CSB-YP025319FIG
CSB-EP025319FIG
CSB-BP025319FIG
CSB-MP025319FIG
CSB-EP025319FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum 40S ribosomal protein S13 (RPS13) CSB-YP020370EWE
CSB-EP020370EWE
CSB-BP020370EWE
CSB-MP020370EWE
CSB-EP020370EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP017878BRJ1
CSB-EP017878BRJ1
CSB-BP017878BRJ1
CSB-MP017878BRJ1
CSB-EP017878BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphobilinogen deaminase (hemC) CSB-YP010524YAS
CSB-EP010524YAS
CSB-BP010524YAS
CSB-MP010524YAS
CSB-EP010524YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flaveria linearis Carbonic anhydrase 1 CSB-YP004364FBP
CSB-EP004364FBP
CSB-BP004364FBP
CSB-MP004364FBP
CSB-EP004364FBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mustela vison Cyclin-dependent kinase inhibitor 1B (CDKN1B) CSB-YP005087MVE
CSB-EP005087MVE
CSB-BP005087MVE
CSB-MP005087MVE
CSB-EP005087MVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Ribose-phosphate pyrophosphokinase 1 (PRS1) CSB-YP018776CZF
CSB-EP018776CZF
CSB-BP018776CZF
CSB-MP018776CZF
CSB-EP018776CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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