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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycoplasma genitalium Methionyl-tRNA formyltransferase (fmt) CSB-YP015150MLN
CSB-EP015150MLN
CSB-BP015150MLN
CSB-MP015150MLN
CSB-EP015150MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium ATP synthase subunit delta (atpH) CSB-YP002355MLN
CSB-EP002355MLN
CSB-BP002355MLN
CSB-MP002355MLN
CSB-EP002355MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Triosephosphate isomerase (tpiA) CSB-YP024102MLN
CSB-EP024102MLN
CSB-BP024102MLN
CSB-MP024102MLN
CSB-EP024102MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium UTP--glucose-1-phosphate uridylyltransferase (galU) CSB-YP025569MLN
CSB-EP025569MLN
CSB-BP025569MLN
CSB-MP025569MLN
CSB-EP025569MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fundulus heteroclitus Glycoprotein hormones alpha chain (cga) CSB-YP005293FDK
CSB-EP005293FDK
CSB-BP005293FDK
CSB-MP005293FDK
CSB-EP005293FDK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Cyclin-A2 (ccna2) CSB-YP004805XBE
CSB-EP004805XBE
CSB-BP004805XBE
CSB-MP004805XBE
CSB-EP004805XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken 40S ribosomal protein S4 (RPS4) CSB-YP020458CH
CSB-EP020458CH
CSB-BP020458CH
CSB-MP020458CH
CSB-EP020458CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Cholesteryl ester transfer protein (CETP) CSB-YP005267MOV
CSB-EP005267MOV
CSB-BP005267MOV
CSB-MP005267MOV
CSB-EP005267MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis 40S ribosomal protein S27 (rps27) CSB-YP020419XBE
CSB-EP020419XBE
CSB-BP020419XBE
CSB-MP020419XBE
CSB-EP020419XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flaveria bidentis Nucleoside diphosphate kinase A CSB-YP015884FBM
CSB-EP015884FBM
CSB-BP015884FBM
CSB-MP015884FBM
CSB-EP015884FBM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meriones unguiculatus Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) CSB-YP010706MRA
CSB-EP010706MRA
CSB-BP010706MRA
CSB-MP010706MRA
CSB-EP010706MRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium- and chloride-dependent betaine transporter (Slc6a12), partial CSB-YP021690RA
CSB-EP021690RA
CSB-BP021690RA
CSB-MP021690RA
CSB-EP021690RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Manduca sexta 40S ribosomal protein S3 (RpS3) CSB-YP020443MPV
CSB-EP020443MPV
CSB-BP020443MPV
CSB-MP020443MPV
CSB-EP020443MPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum 60S ribosomal protein L27a (rpl27a) CSB-YP020214DKK
CSB-EP020214DKK
CSB-BP020214DKK
CSB-MP020214DKK
CSB-EP020214DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss ATP synthase subunit a (mt-atp6), partial CSB-YP015070OEI1
CSB-EP015070OEI1
CSB-BP015070OEI1
CSB-MP015070OEI1
CSB-EP015070OEI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Iron-responsive element-binding protein 2 (IREB2), partial CSB-YP011813HU
CSB-EP011813HU
CSB-BP011813HU
CSB-MP011813HU
CSB-EP011813HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exosome complex component MTR3 (MTR3) CSB-YP007894SVG
CSB-EP007894SVG
CSB-BP007894SVG
CSB-MP007894SVG
CSB-EP007894SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Morone saxatilis Somatotropin (gh) CSB-YP009407MUL
CSB-EP009407MUL
CSB-BP009407MUL
CSB-MP009407MUL
CSB-EP009407MUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ubiquinone biosynthesis protein COQ7 homolog (clk-1) CSB-YP005806CXY
CSB-EP005806CXY
CSB-BP005806CXY
CSB-MP005806CXY
CSB-EP005806CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Biotin--protein ligase (BPL1), partial CSB-YP010518SVG
CSB-EP010518SVG
CSB-BP010518SVG
CSB-MP010518SVG
CSB-EP010518SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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