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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Peflin (PEF1) CSB-YP017771SVG
CSB-EP017771SVG
CSB-BP017771SVG
CSB-MP017771SVG
CSB-EP017771SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exosome complex component RRP46 (RRP46) CSB-YP007893SVG
CSB-EP007893SVG
CSB-BP007893SVG
CSB-MP007893SVG
CSB-EP007893SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin beta chain (TUB2) CSB-YP025318PEW
CSB-EP025318PEW
CSB-BP025318PEW
CSB-MP025318PEW
CSB-EP025318PEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Takifugu rubripes T-complex protein 1 subunit delta (cct4), partial CSB-YP004859TKY
CSB-EP004859TKY
CSB-BP004859TKY
CSB-MP004859TKY
CSB-EP004859TKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin CSB-YP001205TQY
CSB-EP001205TQY
CSB-BP001205TQY
CSB-MP001205TQY
CSB-EP001205TQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydomonas reinhardtii Actin CSB-YP001205DSJ
CSB-EP001205DSJ
CSB-BP001205DSJ
CSB-MP001205DSJ
CSB-EP001205DSJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (SSU72) CSB-YP022729SVG
CSB-EP022729SVG
CSB-BP022729SVG
CSB-MP022729SVG
CSB-EP022729SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae THO complex subunit 2 (THO2), partial CSB-YP023502SVG
CSB-EP023502SVG
CSB-BP023502SVG
CSB-MP023502SVG
CSB-EP023502SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Coatomer subunit alpha (COP1), partial CSB-YP005782SVG
CSB-EP005782SVG
CSB-BP005782SVG
CSB-MP005782SVG
CSB-EP005782SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MVJ
CSB-EP022398MVJ
CSB-BP022398MVJ
CSB-MP022398MVJ
CSB-EP022398MVJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocidura russula Acetylcholine receptor subunit alpha (CHRNA1) CSB-YP005386CSI
CSB-EP005386CSI
CSB-BP005386CSI
CSB-MP005386CSI
CSB-EP005386CSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Cacna1a), partial CSB-YP004397RA
CSB-EP004397RA
CSB-BP004397RA
CSB-MP004397RA
CSB-EP004397RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Septin-2 (Sep2) CSB-YP021025DLU
CSB-EP021025DLU
CSB-BP021025DLU
CSB-MP021025DLU
CSB-EP021025DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Homeobox protein goosecoid (Gsc) CSB-YP009950DLU
CSB-EP009950DLU
CSB-BP009950DLU
CSB-MP009950DLU
CSB-EP009950DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avena sativa T-complex protein 1 subunit epsilon, partial CSB-YP004862DPO
CSB-EP004862DPO
CSB-BP004862DPO
CSB-MP004862DPO
CSB-EP004862DPO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Glucokinase (glcK) CSB-YP009319BRJ
CSB-EP009319BRJ
CSB-BP009319BRJ
CSB-MP009319BRJ
CSB-EP009319BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae Branched-chain-amino-acid aminotransferase (ilvE) CSB-YP002601HTA
CSB-EP002601HTA
CSB-BP002601HTA
CSB-MP002601HTA
CSB-EP002601HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae UBX domain-containing protein 4 (UBX4) CSB-YP025536SVG
CSB-EP025536SVG
CSB-BP025536SVG
CSB-MP025536SVG
CSB-EP025536SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Dolichol-phosphate mannosyltransferase (DPM1), partial CSB-YP007134UBS1
CSB-EP007134UBS1
CSB-BP007134UBS1
CSB-MP007134UBS1
CSB-EP007134UBS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Hydroxymethylglutaryl-CoA synthase (HMGS) CSB-YP010567DOA
CSB-EP010567DOA
CSB-BP010567DOA
CSB-MP010567DOA
CSB-EP010567DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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