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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila melanogaster 40S ribosomal protein S3a (RpS3A) CSB-YP020444DLU
CSB-EP020444DLU
CSB-BP020444DLU
CSB-MP020444DLU
CSB-EP020444DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ubiquitin carboxyl-terminal hydrolase 11 (USP11), partial CSB-YP025698HU
CSB-EP025698HU
CSB-BP025698HU
CSB-MP025698HU
CSB-EP025698HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Protein Wnt-11 (WNT11) CSB-YP026131DXJ
CSB-EP026131DXJ
CSB-BP026131DXJ
CSB-MP026131DXJ
CSB-EP026131DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles arabiensis NADH-ubiquinone oxidoreductase chain 5 (ND5), partial CSB-YP015081AKS
CSB-EP015081AKS
CSB-BP015081AKS
CSB-MP015081AKS
CSB-EP015081AKS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Excitatory amino acid transporter 3 (Slc1a1), partial CSB-YP021432RA
CSB-EP021432RA
CSB-BP021432RA
CSB-MP021432RA
CSB-EP021432RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gap junction alpha-4 protein (gja4), partial CSB-YP009447XBE1
CSB-EP009447XBE1
CSB-BP009447XBE1
CSB-MP009447XBE1
CSB-EP009447XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sparus aurata Progonadoliberin-2 (gnrh2) CSB-YP009636FJX
CSB-EP009636FJX
CSB-BP009636FJX
CSB-MP009636FJX
CSB-EP009636FJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Glycerol-3-phosphate dehydrogenase (glpD), partial CSB-YP009711EZX
CSB-EP009711EZX
CSB-BP009711EZX
CSB-MP009711EZX
CSB-EP009711EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Chymase (CMA1) CSB-YP005599ERJ
CSB-EP005599ERJ
CSB-BP005599ERJ
CSB-MP005599ERJ
CSB-EP005599ERJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Proteasome subunit alpha type-1 (PAF1) CSB-YP018865OFG
CSB-EP018865OFG
CSB-BP018865OFG
CSB-MP018865OFG
CSB-EP018865OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transcription initiation factor IIA subunit 1 (TfIIA-L) CSB-YP009998DLU
CSB-EP009998DLU
CSB-BP009998DLU
CSB-MP009998DLU
CSB-EP009998DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine/threonine-protein phosphatase 5 (Ppp5c) CSB-YP018582RA
CSB-EP018582RA
CSB-BP018582RA
CSB-MP018582RA
CSB-EP018582RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Conserved oligomeric Golgi complex subunit 7 (COG7) CSB-YP005711SVG
CSB-EP005711SVG
CSB-BP005711SVG
CSB-MP005711SVG
CSB-EP005711SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Eukaryotic translation initiation factor 2A (YGR054W), partial CSB-YP007509SVG
CSB-EP007509SVG
CSB-BP007509SVG
CSB-MP007509SVG
CSB-EP007509SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Twinfilin-1 (TWF1) CSB-YP025356SVG
CSB-EP025356SVG
CSB-BP025356SVG
CSB-MP025356SVG
CSB-EP025356SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Conserved oligomeric Golgi complex subunit 2 (COG2) CSB-YP005706SVG
CSB-EP005706SVG
CSB-BP005706SVG
CSB-MP005706SVG
CSB-EP005706SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Homeobox protein goosecoid (gsc) CSB-YP009950DIL
CSB-EP009950DIL
CSB-BP009950DIL
CSB-MP009950DIL
CSB-EP009950DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917DXP
CSB-EP022917DXP
CSB-BP022917DXP
CSB-MP022917DXP
CSB-EP022917DXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bordetella pertussis Superoxide dismutase [Mn] (sodA) CSB-YP022398BUA
CSB-EP022398BUA
CSB-BP022398BUA
CSB-MP022398BUA
CSB-EP022398BUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MLG
CSB-EP022398MLG
CSB-BP022398MLG
CSB-MP022398MLG
CSB-EP022398MLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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