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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio Protein Wnt-8b (wnt8b) CSB-YP026144DIL
CSB-EP026144DIL
CSB-BP026144DIL
CSB-MP026144DIL
CSB-EP026144DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uracil phosphoribosyltransferase homolog CSB-YP025661EXV
CSB-EP025661EXV
CSB-BP025661EXV
CSB-MP025661EXV
CSB-EP025661EXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S2 (rps-2) CSB-YP020393CXY
CSB-EP020393CXY
CSB-BP020393CXY
CSB-MP020393CXY
CSB-EP020393CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 60S ribosomal protein L39 (RPL39) CSB-YP020277ZAX
CSB-EP020277ZAX
CSB-BP020277ZAX
CSB-MP020277ZAX
CSB-EP020277ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pithecia irrorata Hemoglobin subunit epsilon (HBE1) CSB-YP010153EWF
CSB-EP010153EWF
CSB-BP010153EWF
CSB-MP010153EWF
CSB-EP010153EWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera NADP-dependent malic enzyme, partial CSB-YP013634VFQ
CSB-EP013634VFQ
CSB-BP013634VFQ
CSB-MP013634VFQ
CSB-EP013634VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 60S ribosomal protein L11 (RpL11) CSB-YP020115DLU
CSB-EP020115DLU
CSB-BP020115DLU
CSB-MP020115DLU
CSB-EP020115DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum Cytochrome b-c1 complex subunit 8 CSB-YP025673FIG
CSB-EP025673FIG
CSB-BP025673FIG
CSB-MP025673FIG
CSB-EP025673FIG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum 40S ribosomal protein S16 (RPS16) CSB-YP020379GHB
CSB-EP020379GHB
CSB-BP020379GHB
CSB-MP020379GHB
CSB-EP020379GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum 40S ribosomal protein S25 (RPS25) CSB-YP020406SZY
CSB-EP020406SZY
CSB-BP020406SZY
CSB-MP020406SZY
CSB-EP020406SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyclin-dependent kinase 12 (cdtl-7), partial CSB-YP005981CXY
CSB-EP005981CXY
CSB-BP005981CXY
CSB-MP005981CXY
CSB-EP005981CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein SA CSB-YP020485EJJ
CSB-EP020485EJJ
CSB-BP020485EJJ
CSB-MP020485EJJ
CSB-EP020485EJJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 60S ribosomal protein L21 (RPL21) CSB-YP020163HU
CSB-EP020163HU
CSB-BP020163HU
CSB-MP020163HU
CSB-EP020163HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cystathionine beta-synthase (cysB) CSB-YP004589DKK
CSB-EP004589DKK
CSB-BP004589DKK
CSB-MP004589DKK
CSB-EP004589DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA polymerase delta subunit 3 (POL32) CSB-YP018305SVG
CSB-EP018305SVG
CSB-BP018305SVG
CSB-MP018305SVG
CSB-EP018305SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Vacuolar protein-sorting-associated protein 25 (VPS25) CSB-YP025897SVG
CSB-EP025897SVG
CSB-BP025897SVG
CSB-MP025897SVG
CSB-EP025897SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoglucomutase-1 (pp63-1), partial CSB-YP017866ERW
CSB-EP017866ERW
CSB-BP017866ERW
CSB-MP017866ERW
CSB-EP017866ERW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium Thymidylate kinase (tmk) CSB-YP007226MLN
CSB-EP007226MLN
CSB-BP007226MLN
CSB-MP007226MLN
CSB-EP007226MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP017878MLN1
CSB-EP017878MLN1
CSB-BP017878MLN1
CSB-MP017878MLN1
CSB-EP017878MLN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma genitalium DNA topoisomerase 1 (topA), partial CSB-YP024056MLN
CSB-EP024056MLN
CSB-BP024056MLN
CSB-MP024056MLN
CSB-EP024056MLN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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