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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Succinate dehydrogenase cytochrome b560 subunit (SDH3), partial CSB-YP020908DZV1
CSB-EP020908DZV1
CSB-BP020908DZV1
CSB-MP020908DZV1
CSB-EP020908DZV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus M-phase inducer phosphatase 3 (CDC25C) CSB-YP004996MRG
CSB-EP004996MRG
CSB-BP004996MRG
CSB-MP004996MRG
CSB-EP004996MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein mago nashi homolog (mag-1) CSB-YP013364CXY
CSB-EP013364CXY
CSB-BP013364CXY
CSB-MP013364CXY
CSB-EP013364CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio cynocephalus Metalloproteinase inhibitor 1 (TIMP1) CSB-YP023560PBR
CSB-EP023560PBR
CSB-BP023560PBR
CSB-MP023560PBR
CSB-EP023560PBR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica oleracea Asparagine synthetase [glutamine-hydrolyzing], partial CSB-YP002219BWE
CSB-EP002219BWE
CSB-BP002219BWE
CSB-MP002219BWE
CSB-EP002219BWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cuscuta reflexa Cytochrome b5, partial CSB-YP006309CXT1
CSB-EP006309CXT1
CSB-BP006309CXT1
CSB-MP006309CXT1
CSB-EP006309CXT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Toxocara canis 60S ribosomal protein L3 (RPL3) CSB-YP020224THA
CSB-EP020224THA
CSB-BP020224THA
CSB-MP020224THA
CSB-EP020224THA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urechis caupo 40S ribosomal protein S2 (RPS2) CSB-YP020393UAQ
CSB-EP020393UAQ
CSB-BP020393UAQ
CSB-MP020393UAQ
CSB-EP020393UAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae 60S ribosomal protein L38 (RPL38) CSB-YP020275SVG
CSB-EP020275SVG
CSB-BP020275SVG
CSB-MP020275SVG
CSB-EP020275SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Profilin-2 (PRO2) CSB-YP017825TQN
CSB-EP017825TQN
CSB-BP017825TQN
CSB-MP017825TQN
CSB-EP017825TQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Farnesyl pyrophosphate synthase (FPS1) CSB-YP008563KBK
CSB-EP008563KBK
CSB-BP008563KBK
CSB-MP008563KBK
CSB-EP008563KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brassica rapa 40S ribosomal protein S3a (RPS3A) CSB-YP020444BWH
CSB-EP020444BWH
CSB-BP020444BWH
CSB-MP020444BWH
CSB-EP020444BWH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Odontella sinensis Acyl carrier protein (acpP) CSB-YP015636OAK
CSB-EP015636OAK
CSB-BP015636OAK
CSB-MP015636OAK
CSB-EP015636OAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ustilago maydis Lanosterol 14-alpha demethylase (ERG11), partial CSB-YP006459UBS
CSB-EP006459UBS
CSB-BP006459UBS
CSB-MP006459UBS
CSB-EP006459UBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo P2Y purinoceptor 1 (P2RY1), partial CSB-YP017326MQV1
CSB-EP017326MQV1
CSB-BP017326MQV1
CSB-MP017326MQV1
CSB-EP017326MQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken V-type proton ATPase subunit B (ATP6V1B) CSB-YP002398CH
CSB-EP002398CH
CSB-BP002398CH
CSB-MP002398CH
CSB-EP002398CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA replication licensing factor mcm6 (mcm6), partial CSB-YP013596SXV
CSB-EP013596SXV
CSB-BP013596SXV
CSB-MP013596SXV
CSB-EP013596SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Malate dehydrogenase (mdh) CSB-YP013623BRJ
CSB-BP013623BRJ
CSB-MP013623BRJ
CSB-EP013623BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Transcription initiation factor TFIID subunit 6 (Taf6), partial CSB-YP023095DLU
CSB-EP023095DLU
CSB-BP023095DLU
CSB-MP023095DLU
CSB-EP023095DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schistosoma mansoni Signal recognition particle 72 kDa protein (SRP72), partial CSB-YP022677SXQ
CSB-EP022677SXQ
CSB-BP022677SXQ
CSB-MP022677SXQ
CSB-EP022677SXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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