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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Danio rerio Insulin gene enhancer protein isl-1 (isl1) CSB-YP011846DIL
CSB-EP011846DIL
CSB-BP011846DIL
CSB-MP011846DIL
CSB-EP011846DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Fructose-bisphosphate aldolase C (aldoc) CSB-YP001587DQG
CSB-EP001587DQG
CSB-BP001587DQG
CSB-MP001587DQG
CSB-EP001587DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actin-related protein 2 (arx-2) CSB-YP001248CXY
CSB-EP001248CXY
CSB-BP001248CXY
CSB-MP001248CXY
CSB-EP001248CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chondrus crispus Actin (AC) CSB-YP001205CJR
CSB-EP001205CJR
CSB-BP001205CJR
CSB-MP001205CJR
CSB-EP001205CJR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Actin-related protein 5 (ARP5), partial CSB-YP001252SVG
CSB-BP001252SVG
CSB-MP001252SVG
CSB-EP001252SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ferrochelatase (hemH) CSB-YP008579SSQ
CSB-EP008579SSQ
CSB-BP008579SSQ
CSB-MP008579SSQ
CSB-EP008579SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Casein kinase I isoform alpha (CkIalpha) CSB-YP006065DLU
CSB-EP006065DLU
CSB-BP006065DLU
CSB-MP006065DLU
CSB-EP006065DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant T-complex protein 1 subunit eta, partial CSB-YP004871TLT
CSB-EP004871TLT
CSB-BP004871TLT
CSB-MP004871TLT
CSB-EP004871TLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pyrroline-5-carboxylate reductase 2 (proI) CSB-YP019116BRJ
CSB-EP019116BRJ
CSB-BP019116BRJ
CSB-MP019116BRJ
CSB-EP019116BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ADP-ribose pyrophosphatase (nudF) CSB-YP016179BRJ
CSB-EP016179BRJ
CSB-BP016179BRJ
CSB-MP016179BRJ
CSB-EP016179BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Origin recognition complex subunit 1 (ORC1), partial CSB-YP017231SVG
CSB-EP017231SVG
CSB-BP017231SVG
CSB-MP017231SVG
CSB-EP017231SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Origin recognition complex subunit 3 (ORC3), partial CSB-YP017233SVG
CSB-EP017233SVG
CSB-BP017233SVG
CSB-MP017233SVG
CSB-EP017233SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Humicola insolens Protein disulfide-isomerase CSB-YP017342HQN
CSB-EP017342HQN
CSB-BP017342HQN
CSB-MP017342HQN
CSB-EP017342HQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transaldolase (tal) CSB-YP023112MVN
CSB-EP023112MVN
CSB-BP023112MVN
CSB-MP023112MVN
CSB-EP023112MVN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla japonica Atrial natriuretic peptide receptor 2 (npr2), partial CSB-YP016024BZH
CSB-EP016024BZH
CSB-BP016024BZH
CSB-MP016024BZH
CSB-EP016024BZH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Caspase-7 (CASP7) CSB-YP004552MRG
CSB-EP004552MRG
CSB-BP004552MRG
CSB-MP004552MRG
CSB-EP004552MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Argininosuccinate synthase (argG) CSB-YP002234ESP
CSB-EP002234ESP
CSB-BP002234ESP
CSB-MP002234ESP
CSB-EP002234ESP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua Cytochrome c oxidase subunit 3 (mt-co3), partial CSB-YP015074GER1
CSB-EP015074GER1
CSB-BP015074GER1
CSB-MP015074GER1
CSB-EP015074GER1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 60S ribosomal protein L14 (RpL14) CSB-YP020142DLU
CSB-EP020142DLU
CSB-BP020142DLU
CSB-MP020142DLU
CSB-EP020142DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Small ubiquitin-related modifier 1 (SUMO1) CSB-YP022948DOA
CSB-EP022948DOA
CSB-BP022948DOA
CSB-MP022948DOA
CSB-EP022948DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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