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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Gibberella fujikuroi Tubulin beta chain (TUB2) CSB-YP025318GGA
CSB-EP025318GGA
CSB-BP025318GGA
CSB-MP025318GGA
CSB-EP025318GGA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Electron transfer flavoprotein subunit alpha (etfA) CSB-YP007844BVW
CSB-EP007844BVW
CSB-BP007844BVW
CSB-MP007844BVW
CSB-EP007844BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Amyloid beta A4 protein (APP), partial CSB-YP001950MOV1
CSB-EP001950MOV1
CSB-BP001950MOV1
CSB-MP001950MOV1
CSB-EP001950MOV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398MVQ
CSB-EP022398MVQ
CSB-BP022398MVQ
CSB-MP022398MVQ
CSB-EP022398MVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398NDY
CSB-EP022398NDY
CSB-BP022398NDY
CSB-MP022398NDY
CSB-EP022398NDY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Superoxide dismutase [Mn] (sodA) CSB-YP022398YAQ
CSB-EP022398YAQ
CSB-BP022398YAQ
CSB-MP022398YAQ
CSB-EP022398YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca radiata Zona pellucida sperm-binding protein 3 (ZP3) CSB-YP027121MOY
CSB-EP027121MOY
CSB-BP027121MOY
CSB-MP027121MOY
CSB-EP027121MOY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana benthamiana Squalene synthase, partial CSB-YP008562NDG1
CSB-EP008562NDG1
CSB-BP008562NDG1
CSB-MP008562NDG1
CSB-EP008562NDG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Exosome complex component CSL4 (CSL4) CSB-YP007888SVG
CSB-EP007888SVG
CSB-BP007888SVG
CSB-MP007888SVG
CSB-EP007888SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erinaceus concolor Acetylcholine receptor subunit alpha (CHRNA1) CSB-YP005386EGF
CSB-EP005386EGF
CSB-BP005386EGF
CSB-MP005386EGF
CSB-EP005386EGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Wee1-like protein kinase (wee) CSB-YP026084DLU
CSB-EP026084DLU
CSB-BP026084DLU
CSB-MP026084DLU
CSB-EP026084DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster DNA polymerase delta catalytic subunit (DNApol-delta), partial CSB-YP018303DLU
CSB-EP018303DLU
CSB-BP018303DLU
CSB-MP018303DLU
CSB-EP018303DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Superoxide dismutase [Mn] (sodA), partial CSB-YP022398BRJ
CSB-EP022398BRJ
CSB-BP022398BRJ
CSB-MP022398BRJ
CSB-EP022398BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tubulin gamma-1 chain CSB-YP025334EPN
CSB-EP025334EPN
CSB-BP025334EPN
CSB-MP025334EPN
CSB-EP025334EPN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila busckii Superoxide dismutase [Cu-Zn] (Sod) CSB-YP022397DFU
CSB-EP022397DFU
CSB-BP022397DFU
CSB-MP022397DFU
CSB-EP022397DFU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Potassium-transporting ATPase alpha chain 2 (Atp12a), partial CSB-YP002316RA
CSB-EP002316RA
CSB-BP002316RA
CSB-MP002316RA
CSB-EP002316RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Small nuclear ribonucleoprotein F (SMX3) CSB-YP022338SVG
CSB-EP022338SVG
CSB-BP022338SVG
CSB-MP022338SVG
CSB-EP022338SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Tyrosinase (tyr), partial CSB-YP025394OFH1
CSB-EP025394OFH1
CSB-BP025394OFH1
CSB-MP025394OFH1
CSB-EP025394OFH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Epidermal growth factor receptor (EGFR) CSB-YP007479MOW
CSB-EP007479MOW
CSB-BP007479MOW
CSB-MP007479MOW
CSB-EP007479MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077GER
CSB-EP015077GER
CSB-BP015077GER
CSB-MP015077GER
CSB-EP015077GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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