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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptomyces coelicolor Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558FOB
CSB-EP001558FOB
CSB-BP001558FOB
CSB-MP001558FOB
CSB-EP001558FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum lycopersicum Inositol monophosphatase 2 (IMP2) CSB-YP011695SZY
CSB-EP011695SZY
CSB-BP011695SZY
CSB-MP011695SZY
CSB-EP011695SZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudopleuronectes americanus Solute carrier family 12 member 3 (slc12a3), partial CSB-YP021384FGV
CSB-EP021384FGV
CSB-BP021384FGV
CSB-MP021384FGV
CSB-EP021384FGV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster 26S proteasome non-ATPase regulatory subunit 4 (Pros54) CSB-YP018908DLU
CSB-EP018908DLU
CSB-BP018908DLU
CSB-MP018908DLU
CSB-EP018908DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Microsomal triglyceride transfer protein large subunit (MTTP), partial CSB-YP015209MRG
CSB-EP015209MRG
CSB-BP015209MRG
CSB-MP015209MRG
CSB-EP015209MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gadus morhua NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079GER
CSB-EP015079GER
CSB-BP015079GER
CSB-MP015079GER
CSB-EP015079GER-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus 40S ribosomal protein S27 (RPS27) CSB-YP020419HWO
CSB-EP020419HWO
CSB-BP020419HWO
CSB-MP020419HWO
CSB-EP020419HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis DNA replication licensing factor mcm2 (mcm2), partial CSB-YP013590XBE
CSB-EP013590XBE
CSB-BP013590XBE
CSB-MP013590XBE
CSB-EP013590XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Eukaryotic translation initiation factor 1A (tif11) CSB-YP007507SXV
CSB-EP007507SXV
CSB-BP007507SXV
CSB-MP007507SXV
CSB-EP007507SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Porphobilinogen deaminase (hemC) CSB-YP010524HUV
CSB-EP010524HUV
CSB-BP010524HUV
CSB-MP010524HUV
CSB-EP010524HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bufo arenarum Galectin-1 CSB-YP012882BNF
CSB-EP012882BNF
CSB-BP012882BNF
CSB-MP012882BNF
CSB-EP012882BNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis E3 ubiquitin-protein ligase Mdm2 (mdm2) CSB-YP013626XBE
CSB-EP013626XBE
CSB-BP013626XBE
CSB-MP013626XBE
CSB-EP013626XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onobrychis viciifolia Eukaryotic translation initiation factor 1A CSB-YP007507OEJ
CSB-EP007507OEJ
CSB-BP007507OEJ
CSB-MP007507OEJ
CSB-EP007507OEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Ribosome-recycling factor (frr) CSB-YP014941HUV
CSB-EP014941HUV
CSB-BP014941HUV
CSB-MP014941HUV
CSB-EP014941HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Cellular tumor antigen p53 (TP53) CSB-YP024077MOV
CSB-EP024077MOV
CSB-BP024077MOV
CSB-MP024077MOV
CSB-EP024077MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844GGZ1
CSB-EP004844GGZ1
CSB-BP004844GGZ1
CSB-MP004844GGZ1
CSB-EP004844GGZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rangifer tarandus Melanocyte-stimulating hormone receptor (MC1R), partial CSB-YP013558RBL1
CSB-EP013558RBL1
CSB-BP013558RBL1
CSB-MP013558RBL1
CSB-EP013558RBL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Papio hamadryas Proteinase-activated receptor 1 (F2R), partial CSB-YP007924ERJ1
CSB-EP007924ERJ1
CSB-BP007924ERJ1
CSB-MP007924ERJ1
CSB-EP007924ERJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spalax ehrenbergi 5-hydroxytryptamine receptor 1B (HTR1B), partial CSB-YP010882FJV1
CSB-EP010882FJV1
CSB-BP010882FJV1
CSB-MP010882FJV1
CSB-EP010882FJV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida maltosa Galactokinase (GAL1) CSB-YP009198CZJ
CSB-EP009198CZJ
CSB-BP009198CZJ
CSB-MP009198CZJ
CSB-EP009198CZJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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