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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila yakuba 60S ribosomal protein L17 (RpL17) CSB-YP744317DMR
CSB-EP744317DMR
CSB-BP744317DMR
CSB-MP744317DMR
CSB-EP744317DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Prostaglandin D2 receptor 2 (Ptgdr2), partial CSB-YP744318RA1
CSB-EP744318RA1
CSB-BP744318RA1
CSB-MP744318RA1
CSB-EP744318RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chaperone protein dnaJ 39 (ATJ39) CSB-YP744319DOA
CSB-EP744319DOA
CSB-BP744319DOA
CSB-MP744319DOA
CSB-EP744319DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Opistophthalmus carinatus Potassium channel toxin alpha-KTx 6.10 CSB-YP744320OBW
CSB-EP744320OBW
CSB-BP744320OBW
CSB-MP744320OBW
CSB-EP744320OBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Zinc finger protein GLIS3 (Glis3) CSB-YP744321MO
CSB-EP744321MO
CSB-BP744321MO
CSB-MP744321MO
CSB-EP744321MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Galactose-3-O-sulfotransferase 2 (Gal3st2), partial CSB-YP744322MO
CSB-EP744322MO
CSB-BP744322MO
CSB-MP744322MO
CSB-EP744322MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-YP744324IDG
CSB-EP744324IDG
CSB-BP744324IDG
CSB-MP744324IDG
CSB-EP744324IDG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Nuclear export protein (NS) CSB-YP744325IHW
CSB-EP744325IHW
CSB-BP744325IHW
CSB-MP744325IHW
CSB-EP744325IHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Non-structural protein 1 (NS) CSB-YP744326IGK
CSB-EP744326IGK
CSB-BP744326IGK
CSB-MP744326IGK
CSB-EP744326IGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Matrix protein 1 (M) CSB-YP744327IHW
CSB-EP744327IHW
CSB-BP744327IHW
CSB-MP744327IHW
CSB-EP744327IHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP744328IAZ
CSB-EP744328IAZ
CSB-BP744328IAZ
CSB-MP744328IAZ
CSB-EP744328IAZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza A virus Neuraminidase (NA), partial CSB-YP744329ICG
CSB-EP744329ICG
CSB-BP744329ICG
CSB-MP744329ICG
CSB-EP744329ICG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Cytochrome P450 2C54 (Cyp2c54) CSB-YP744330MO
CSB-EP744330MO
CSB-BP744330MO
CSB-MP744330MO
CSB-EP744330MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L5 (rplE) CSB-YP744331SJV
CSB-EP744331SJV
CSB-BP744331SJV
CSB-MP744331SJV
CSB-EP744331SJV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Fructokinase-2 (FRK2) CSB-YP744332ZAX
CSB-EP744332ZAX
CSB-BP744332ZAX
CSB-MP744332ZAX
CSB-EP744332ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Myeloid differentiation primary response protein MyD88 (Myd88) CSB-YP744333RA
CSB-EP744333RA
CSB-BP744333RA
CSB-MP744333RA
CSB-EP744333RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human DnaJ homolog subfamily C member 14 (DNAJC14), partial CSB-YP744334HU1
CSB-EP744334HU1
CSB-BP744334HU1
CSB-MP744334HU1
CSB-EP744334HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uroderma magnirostrum Cytochrome b (MT-CYB), partial CSB-YP744335UAAH
CSB-EP744335UAAH
CSB-BP744335UAAH
CSB-MP744335UAAH
CSB-EP744335UAAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse TD and POZ domain-containing protein 4 (Tdpoz4) CSB-YP744336MO
CSB-EP744336MO
CSB-BP744336MO
CSB-MP744336MO
CSB-EP744336MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ectophylla alba Cytochrome b (MT-CYB), partial CSB-YP744337EAAP
CSB-EP744337EAAP
CSB-BP744337EAAP
CSB-MP744337EAAP
CSB-EP744337EAAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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