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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arthroderma otae Extracellular metalloproteinase 4 (MEP4) CSB-YP744297AVB
CSB-EP744297AVB
CSB-BP744297AVB
CSB-MP744297AVB
CSB-EP744297AVB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog Hypoxanthine-guanine phosphoribosyltransferase (HPRT1) CSB-YP744298DO
CSB-EP744298DO
CSB-BP744298DO
CSB-MP744298DO
CSB-EP744298DO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lagothrix lagotricha Hemoglobin subunit beta (HBB) CSB-YP744299LNL
CSB-EP744299LNL
CSB-BP744299LNL
CSB-MP744299LNL
CSB-EP744299LNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Protein VP3, partial CSB-YP744300ROB
CSB-EP744300ROB
CSB-BP744300ROB
CSB-MP744300ROB
CSB-EP744300ROB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Akodon montensis Cytochrome b (MT-CYB), partial CSB-YP744301AFAG
CSB-EP744301AFAG
CSB-BP744301AFAG
CSB-MP744301AFAG
CSB-EP744301AFAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces kambucha Mating-type M-specific polypeptide Mi (matMi) CSB-YP744302SFAV
CSB-EP744302SFAV
CSB-BP744302SFAV
CSB-MP744302SFAV
CSB-EP744302SFAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Polypeptide N-acetylgalactosaminyltransferase 2 (pgant2), partial CSB-YP744303DLU
CSB-EP744303DLU
CSB-BP744303DLU
CSB-MP744303DLU
CSB-EP744303DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Protocadherin-11 X-linked (PCDH11X), partial CSB-YP744304EXP
CSB-EP744304EXP
CSB-BP744304EXP
CSB-MP744304EXP
CSB-EP744304EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aravan virus Phosphoprotein (P) CSB-YP744305AFAH
CSB-EP744305AFAH
CSB-BP744305AFAH
CSB-MP744305AFAH
CSB-EP744305AFAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Claviceps purpurea Tryptophan dimethylallyltransferase 1 (dmaW1) CSB-YP744306CLL
CSB-EP744306CLL
CSB-BP744306CLL
CSB-MP744306CLL
CSB-EP744306CLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acremonium coenophialum Tryptophan dimethylallyltransferase 1 (dmaW1) CSB-YP744307ACF
CSB-EP744307ACF
CSB-BP744307ACF
CSB-MP744307ACF
CSB-EP744307ACF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss 15 kDa selenoprotein (41532) CSB-YP744308OEI
CSB-EP744308OEI
CSB-BP744308OEI
CSB-MP744308OEI
CSB-EP744308OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bitis arietans C-type lectin 5 CSB-YP744309BTG
CSB-EP744309BTG
CSB-BP744309BTG
CSB-MP744309BTG
CSB-EP744309BTG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana WUSCHEL-related homeobox 9 (WOX9) CSB-YP744310DOA
CSB-EP744310DOA
CSB-BP744310DOA
CSB-MP744310DOA
CSB-EP744310DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Kin of IRRE-like protein 1 (Kirrel), partial CSB-YP744311RA
CSB-EP744311RA
CSB-BP744311RA
CSB-MP744311RA
CSB-EP744311RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse One cut domain family member 2 (Onecut2) CSB-YP744312MO
CSB-EP744312MO
CSB-BP744312MO
CSB-MP744312MO
CSB-EP744312MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Picea sitchensis Pinene synthase, chloroplastic, partial CSB-YP744313EVO
CSB-EP744313EVO
CSB-BP744313EVO
CSB-MP744313EVO
CSB-EP744313EVO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glycophorin-C (Gypc), partial CSB-YP744314RA
CSB-EP744314RA
CSB-BP744314RA
CSB-MP744314RA
CSB-EP744314RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba 40S ribosomal protein S11 (RpS11) CSB-YP744315DMR
CSB-EP744315DMR
CSB-BP744315DMR
CSB-MP744315DMR
CSB-EP744315DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba 40S ribosomal protein S15a (RpS15Aa) CSB-YP744316DMR
CSB-EP744316DMR
CSB-BP744316DMR
CSB-MP744316DMR
CSB-EP744316DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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