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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Oryza sativa subsp. japonica Probable auxin efflux carrier component 4 (PIN4), partial CSB-YP757927OFG
CSB-EP757927OFG
CSB-BP757927OFG
CSB-MP757927OFG
CSB-EP757927OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable uridine nucleosidase 1 (URH1) CSB-YP757928OFG
CSB-EP757928OFG
CSB-BP757928OFG
CSB-MP757928OFG
CSB-EP757928OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Pre-mRNA-splicing factor SLU7 (Os08g0127700, LOC_Os08g03390), partial CSB-YP757929OFG
CSB-EP757929OFG
CSB-BP757929OFG
CSB-MP757929OFG
CSB-EP757929OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica Probable succinyl-CoA ligase [ADP-forming] subunit alpha, mitochondrial (Os07g0577700, LOC_Os07g38970) CSB-YP757930OFG
CSB-EP757930OFG
CSB-BP757930OFG
CSB-MP757930OFG
CSB-EP757930OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Delphilin (grid2ip), partial CSB-YP757931DIL
CSB-EP757931DIL
CSB-BP757931DIL
CSB-MP757931DIL
CSB-EP757931DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protease serine 11A (TMPRSS11A), partial CSB-YP757932HU
CSB-MP757932HU
CSB-EP757932HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein ZNF783 (ZNF783) CSB-YP757933HU
CSB-EP757933HU
CSB-BP757933HU
CSB-MP757933HU
CSB-EP757933HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human SPOC domain-containing protein 1 (SPOCD1), partial CSB-YP757934HU
CSB-EP757934HU
CSB-BP757934HU
CSB-MP757934HU
CSB-EP757934HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein encoded by LINC00322 (LINC00322) CSB-YP757935HU
CSB-EP757935HU
CSB-BP757935HU
CSB-MP757935HU
CSB-EP757935HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative zinc finger protein 66 (ZNF66P) CSB-YP757936HU
CSB-EP757936HU
CSB-BP757936HU
CSB-MP757936HU
CSB-EP757936HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative protein dpy-19 homolog 2-like 2 (DPY19L2P2), partial CSB-YP757937HU1
CSB-EP757937HU1
CSB-BP757937HU1
CSB-MP757937HU1
CSB-EP757937HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NF-X1-type zinc finger protein NFXL1 (NFXL1), partial CSB-YP757938HU
CSB-EP757938HU
CSB-BP757938HU
CSB-MP757938HU
CSB-EP757938HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human FYVE, RhoGEF and PH domain-containing protein 5 (FGD5), partial CSB-YP757939HU
CSB-EP757939HU
CSB-BP757939HU
CSB-MP757939HU
CSB-EP757939HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human RNA-binding protein 44 (RBM44), partial CSB-YP757940HU
CSB-EP757940HU
CSB-BP757940HU
CSB-MP757940HU
CSB-EP757940HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Diacylglycerol O-acyltransferase 2-like protein 6 (DGAT2L6), partial CSB-YP757941HU1
CSB-EP757941HU1
CSB-BP757941HU1
CSB-MP757941HU1
CSB-EP757941HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Testis-expressed protein 2 (Tex2), partial CSB-YP757942MO
CSB-EP757942MO
CSB-BP757942MO
CSB-MP757942MO
CSB-EP757942MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Inhibitor of Bruton tyrosine kinase (Ibtk), partial CSB-YP757943MO
CSB-EP757943MO
CSB-BP757943MO
CSB-MP757943MO
CSB-EP757943MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Lysine-specific demethylase 3B (Kdm3b), partial CSB-YP757944MO
CSB-EP757944MO
CSB-BP757944MO
CSB-MP757944MO
CSB-EP757944MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine/threonine-protein kinase TAO2 (Taok2), partial CSB-YP757945MO
CSB-EP757945MO
CSB-BP757945MO
CSB-MP757945MO
CSB-EP757945MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Spermatogenesis-associated protein 31D1 (SPATA31D1), partial CSB-YP757946HU
CSB-EP757946HU
CSB-BP757946HU
CSB-MP757946HU
CSB-EP757946HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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