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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus epidermidis UPF0340 protein SE_1711 (SE_1711) CSB-YP807603SLD
CSB-EP807603SLD
CSB-BP807603SLD
CSB-MP807603SLD
CSB-EP807603SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Antitoxin MazE (mazE) CSB-YP807604SLD
CSB-EP807604SLD
CSB-BP807604SLD
CSB-MP807604SLD
CSB-EP807604SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Glutamyl-tRNA (Gln) amidotransferase subunit A (gatA) CSB-YP807605SLD
CSB-EP807605SLD
CSB-BP807605SLD
CSB-MP807605SLD
CSB-EP807605SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0374 protein SE_1551 (SE_1551) CSB-YP807606SLD
CSB-EP807606SLD
CSB-BP807606SLD
CSB-MP807606SLD
CSB-EP807606SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative membrane protein insertion efficiency factor (SE_1462) CSB-YP807607SLD
CSB-EP807607SLD
CSB-BP807607SLD
CSB-MP807607SLD
CSB-EP807607SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0354 protein SE_1416 (SE_1416) CSB-YP807608SLD
CSB-EP807608SLD
CSB-BP807608SLD
CSB-MP807608SLD
CSB-EP807608SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Pyruvate kinase (pyk), partial CSB-YP807609SLD
CSB-EP807609SLD
CSB-BP807609SLD
CSB-MP807609SLD
CSB-EP807609SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 50S ribosomal protein L21 (rplU) CSB-YP807610SLD
CSB-EP807610SLD
CSB-BP807610SLD
CSB-MP807610SLD
CSB-EP807610SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis GTP pyrophosphokinase (relA), partial CSB-YP807611SLD
CSB-EP807611SLD
CSB-BP807611SLD
CSB-MP807611SLD
CSB-EP807611SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative Holliday junction resolvase (SE_1299) CSB-YP807612SLD
CSB-EP807612SLD
CSB-BP807612SLD
CSB-MP807612SLD
CSB-EP807612SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP807613SLD
CSB-EP807613SLD
CSB-BP807613SLD
CSB-MP807613SLD
CSB-EP807613SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 50S ribosomal protein L33 1 (rpmG1) CSB-YP807614SLD
CSB-EP807614SLD
CSB-BP807614SLD
CSB-MP807614SLD
CSB-EP807614SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0403 protein SE_1195 (SE_1195) CSB-YP807615SLD
CSB-EP807615SLD
CSB-BP807615SLD
CSB-MP807615SLD
CSB-EP807615SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0398 protein SE_1135 (SE_1135) CSB-YP807616SLD
CSB-EP807616SLD
CSB-BP807616SLD
CSB-MP807616SLD
CSB-EP807616SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized hydrolase SE_1078 (SE_1078) CSB-YP807617SLD
CSB-EP807617SLD
CSB-BP807617SLD
CSB-MP807617SLD
CSB-EP807617SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis DNA topoisomerase 4 subunit B (parE), partial CSB-YP807618SLD
CSB-EP807618SLD
CSB-BP807618SLD
CSB-MP807618SLD
CSB-EP807618SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Translation initiation factor IF-2 (infB), partial CSB-YP807619SLD
CSB-EP807619SLD
CSB-BP807619SLD
CSB-MP807619SLD
CSB-EP807619SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Ribosome-recycling factor (frr) CSB-YP807620SLD
CSB-EP807620SLD
CSB-BP807620SLD
CSB-MP807620SLD
CSB-EP807620SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized protein SE_0901 (SE_0901), partial CSB-YP807621SLD
CSB-EP807621SLD
CSB-BP807621SLD
CSB-MP807621SLD
CSB-EP807621SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Protein MraZ (mraZ) CSB-YP807622SLD
CSB-EP807622SLD
CSB-BP807622SLD
CSB-MP807622SLD
CSB-EP807622SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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