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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus epidermidis Putative peptidyl-prolyl cis-trans isomerase (SE_0648) CSB-YP809391SLD
CSB-EP809391SLD
CSB-BP809391SLD
CSB-MP809391SLD
CSB-EP809391SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Methionine import ATP-binding protein MetN 1 (metN1) CSB-YP809392SLD
CSB-EP809392SLD
CSB-BP809392SLD
CSB-MP809392SLD
CSB-EP809392SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis NADPH-dependent 7-cyano-7-deazaguanine reductase (queF) CSB-YP809393SLD
CSB-EP809393SLD
CSB-BP809393SLD
CSB-MP809393SLD
CSB-EP809393SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0178 protein SE_0451 (SE_0451) CSB-YP809394SLD
CSB-EP809394SLD
CSB-BP809394SLD
CSB-MP809394SLD
CSB-EP809394SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Putative antiporter subunit mnhG2 (mnhG2), partial CSB-YP809395SLD1
CSB-EP809395SLD1
CSB-BP809395SLD1
CSB-MP809395SLD1
CSB-EP809395SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 30S ribosomal protein S12 (rpsL) CSB-YP809396SLD
CSB-EP809396SLD
CSB-BP809396SLD
CSB-MP809396SLD
CSB-EP809396SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis UPF0312 protein SE_0264 (SE_0264) CSB-YP809397SLD
CSB-EP809397SLD
CSB-BP809397SLD
CSB-MP809397SLD
CSB-EP809397SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis 6-carboxyhexanoate--CoA ligase (bioW) CSB-YP809398SLD
CSB-EP809398SLD
CSB-BP809398SLD
CSB-MP809398SLD
CSB-EP809398SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Uncharacterized lipoprotein SE_0142 (SE_0142) CSB-YP809399SLD
CSB-EP809399SLD
CSB-BP809399SLD
CSB-MP809399SLD
CSB-EP809399SLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus epidermidis Sensor protein kinase walK (walK), partial CSB-YP809400SLD1
CSB-EP809400SLD1
CSB-BP809400SLD1
CSB-MP809400SLD1
CSB-EP809400SLD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis UPF0342 protein OB1136 (OB1136) CSB-YP809401OAB
CSB-EP809401OAB
CSB-BP809401OAB
CSB-MP809401OAB
CSB-EP809401OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis 2-oxoglutarate dehydrogenase E1 component (odhA), partial CSB-YP809402OAB
CSB-EP809402OAB
CSB-BP809402OAB
CSB-MP809402OAB
CSB-EP809402OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Gamma-glutamyl phosphate reductase (proA) CSB-YP809403OAB
CSB-EP809403OAB
CSB-BP809403OAB
CSB-MP809403OAB
CSB-EP809403OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oceanobacillus iheyensis Probable spermidine synthase (speE), partial CSB-YP809404OAB
CSB-EP809404OAB
CSB-BP809404OAB
CSB-MP809404OAB
CSB-EP809404OAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable N-acetylneuraminic acid outer membrane channel protein NanC (nanC), partial CSB-YP809405EGX
CSB-EP809405EGX
CSB-BP809405EGX
CSB-MP809405EGX
CSB-EP809405EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant N-acetylneuraminate epimerase 1 (nanM1) CSB-YP809406EGX
CSB-EP809406EGX
CSB-BP809406EGX
CSB-MP809406EGX
CSB-EP809406EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ecotin (eco) CSB-YP809407EGX
CSB-EP809407EGX
CSB-BP809407EGX
CSB-MP809407EGX
CSB-EP809407EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Outer-membrane lipoprotein LolB (lolB) CSB-YP809408EGX
CSB-EP809408EGX
CSB-BP809408EGX
CSB-MP809408EGX
CSB-EP809408EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cation efflux system protein CusB (cusB) CSB-YP809409EGX
CSB-EP809409EGX
CSB-BP809409EGX
CSB-MP809409EGX
CSB-EP809409EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0412 protein yaaI (yaaI) CSB-YP809410EGX
CSB-EP809410EGX
CSB-BP809410EGX
CSB-MP809410EGX
CSB-EP809410EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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