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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phosphopentomutase (deoB) CSB-YP809511VFI
CSB-EP809511VFI
CSB-BP809511VFI
CSB-MP809511VFI
CSB-EP809511VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-2 (infB), partial CSB-YP809512VFI
CSB-EP809512VFI
CSB-BP809512VFI
CSB-MP809512VFI
CSB-EP809512VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tyrosine--tRNA ligase 2 (tyrS2) CSB-YP809513VFI
CSB-EP809513VFI
CSB-BP809513VFI
CSB-MP809513VFI
CSB-EP809513VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine--tRNA ligase (alaS), partial CSB-YP809514VFI
CSB-EP809514VFI
CSB-BP809514VFI
CSB-MP809514VFI
CSB-EP809514VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enolase (eno) CSB-YP809515VFI
CSB-EP809515VFI
CSB-BP809515VFI
CSB-MP809515VFI
CSB-EP809515VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GTPase Era (era) CSB-YP809516VFI
CSB-EP809516VFI
CSB-BP809516VFI
CSB-MP809516VFI
CSB-EP809516VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0149 protein VV1_1551 (VV1_1551) CSB-YP809517VFI
CSB-EP809517VFI
CSB-BP809517VFI
CSB-MP809517VFI
CSB-EP809517VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA (guanine-N (7)-)-methyltransferase (trmB) CSB-YP809518VFI
CSB-EP809518VFI
CSB-BP809518VFI
CSB-MP809518VFI
CSB-EP809518VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine deiminase (arcA) CSB-YP809519VFI
CSB-EP809519VFI
CSB-BP809519VFI
CSB-MP809519VFI
CSB-EP809519VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maf-like protein VV1_1452 (VV1_1452) CSB-YP809520VFI
CSB-EP809520VFI
CSB-BP809520VFI
CSB-MP809520VFI
CSB-EP809520VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alanine racemase 1 (alr1) CSB-YP809521VFI
CSB-EP809521VFI
CSB-BP809521VFI
CSB-MP809521VFI
CSB-EP809521VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shikimate kinase (aroK) CSB-YP809522VFI
CSB-EP809522VFI
CSB-BP809522VFI
CSB-MP809522VFI
CSB-EP809522VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division protein ZapB (zapB) CSB-YP809523VFI
CSB-EP809523VFI
CSB-BP809523VFI
CSB-MP809523VFI
CSB-EP809523VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S7 (rpsG) CSB-YP809524VFI
CSB-EP809524VFI
CSB-BP809524VFI
CSB-MP809524VFI
CSB-EP809524VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 23S rRNA (guanosine-2'-O-)-methyltransferase RlmB (rlmB) CSB-YP809525VFI
CSB-EP809525VFI
CSB-BP809525VFI
CSB-MP809525VFI
CSB-EP809525VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP809526VFI
CSB-EP809526VFI
CSB-BP809526VFI
CSB-MP809526VFI
CSB-EP809526VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-coenzyme A synthetase (acsA), partial CSB-YP809527VFI
CSB-EP809527VFI
CSB-BP809527VFI
CSB-MP809527VFI
CSB-EP809527VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP809528VFI
CSB-EP809528VFI
CSB-BP809528VFI
CSB-MP809528VFI
CSB-EP809528VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP809529VFI
CSB-EP809529VFI
CSB-BP809529VFI
CSB-MP809529VFI
CSB-EP809529VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable chorismate--pyruvate lyase (ubiC) CSB-YP809530VFI
CSB-EP809530VFI
CSB-BP809530VFI
CSB-MP809530VFI
CSB-EP809530VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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