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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Methylenetetrahydrofolate--tRNA- (uracil-5-)-methyltransferase TrmFO (trmFO) CSB-YP807923SMI
CSB-EP807923SMI
CSB-BP807923SMI
CSB-MP807923SMI
CSB-EP807923SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP807924SMI
CSB-EP807924SMI
CSB-BP807924SMI
CSB-MP807924SMI
CSB-EP807924SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP807925SMI
CSB-EP807925SMI
CSB-BP807925SMI
CSB-MP807925SMI
CSB-EP807925SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine synthase (metK) CSB-YP807926SMI
CSB-EP807926SMI
CSB-BP807926SMI
CSB-MP807926SMI
CSB-EP807926SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoenolpyruvate carboxylase (ppc), partial CSB-YP807927SMI
CSB-EP807927SMI
CSB-BP807927SMI
CSB-MP807927SMI
CSB-EP807927SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized RNA methyltransferase SAG0633 (SAG0633) CSB-YP807928SMI
CSB-EP807928SMI
CSB-BP807928SMI
CSB-MP807928SMI
CSB-EP807928SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP807929SMI
CSB-EP807929SMI
CSB-BP807929SMI
CSB-MP807929SMI
CSB-EP807929SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Valine--tRNA ligase (valS), partial CSB-YP807930SMI
CSB-EP807930SMI
CSB-BP807930SMI
CSB-MP807930SMI
CSB-EP807930SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP807931SMI
CSB-EP807931SMI
CSB-BP807931SMI
CSB-MP807931SMI
CSB-EP807931SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP807932SMI
CSB-EP807932SMI
CSB-BP807932SMI
CSB-MP807932SMI
CSB-EP807932SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sensory transduction protein lytR (lytR) CSB-YP807933SMI
CSB-EP807933SMI
CSB-BP807933SMI
CSB-MP807933SMI
CSB-EP807933SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP807934SMI
CSB-EP807934SMI
CSB-BP807934SMI
CSB-MP807934SMI
CSB-EP807934SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Heat-inducible transcription repressor HrcA (hrcA) CSB-YP807935SMI
CSB-EP807935SMI
CSB-BP807935SMI
CSB-MP807935SMI
CSB-EP807935SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP807936SMI
CSB-EP807936SMI
CSB-BP807936SMI
CSB-MP807936SMI
CSB-EP807936SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L14 (rplN) CSB-YP807937SMI
CSB-EP807937SMI
CSB-BP807937SMI
CSB-MP807937SMI
CSB-EP807937SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP807938SMI
CSB-EP807938SMI
CSB-BP807938SMI
CSB-MP807938SMI
CSB-EP807938SMI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arginine deiminase (arcA) CSB-YP807939SMH
CSB-EP807939SMH
CSB-BP807939SMH
CSB-MP807939SMH
CSB-EP807939SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L9 (rplI) CSB-YP807940SMH
CSB-EP807940SMH
CSB-BP807940SMH
CSB-MP807940SMH
CSB-EP807940SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Galactose-6-phosphate isomerase subunit lacA 2 (lacA2) CSB-YP807941SMH
CSB-EP807941SMH
CSB-BP807941SMH
CSB-MP807941SMH
CSB-EP807941SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proline--tRNA ligase (proS), partial CSB-YP807942SMH
CSB-EP807942SMH
CSB-BP807942SMH
CSB-MP807942SMH
CSB-EP807942SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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