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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Chicken Inosine triphosphate pyrophosphatase (ITPA) CSB-YP011907CH
CSB-EP011907CH
CSB-BP011907CH
CSB-MP011907CH
CSB-EP011907CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig AP2-associated protein kinase 1 (AAK1), partial CSB-YP001020PI
CSB-EP001020PI
CSB-BP001020PI
CSB-MP001020PI
CSB-EP001020PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Acetyl-CoA carboxylase 2 (ACC2), partial CSB-YP001120DOA
CSB-EP001120DOA
CSB-BP001120DOA
CSB-MP001120DOA
CSB-EP001120DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vitis vinifera Inosine triphosphate pyrophosphatase (VIT_05s0051g00580) CSB-YP011907VFQ
CSB-EP011907VFQ
CSB-BP011907VFQ
CSB-MP011907VFQ
CSB-EP011907VFQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica U1 small nuclear ribonucleoprotein C (SNRPC) CSB-YP022331MUE
CSB-EP022331MUE
CSB-BP022331MUE
CSB-MP022331MUE
CSB-EP022331MUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio E3 ubiquitin-protein ligase HACE1 (hace1), partial CSB-YP010115DIL
CSB-EP010115DIL
CSB-BP010115DIL
CSB-MP010115DIL
CSB-EP010115DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Monocarboxylate transporter 6 (Slc16a5), partial CSB-YP021412MO
CSB-EP021412MO
CSB-BP021412MO
CSB-MP021412MO
CSB-EP021412MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Alpha-1-antitrypsin (SERPINA1) CSB-YP021053DSU
CSB-EP021053DSU
CSB-BP021053DSU
CSB-MP021053DSU
CSB-EP021053DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) CSB-YP011092HU
CSB-BP011092HU
CSB-MP011092HU
CSB-EP011092HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nucleolar protein 56 (NOP56) CSB-YP015939HU
CSB-EP015939HU
CSB-BP015939HU
CSB-MP015939HU
CSB-EP015939HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S acidic ribosomal protein P1 (rpl-21) CSB-YP020340OFK
CSB-EP020340OFK
CSB-BP020340OFK
CSB-MP020340OFK
CSB-EP020340OFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eukaryotic elongation factor 2 kinase (efk-1), partial CSB-YP007435CXY
CSB-EP007435CXY
CSB-BP007435CXY
CSB-MP007435CXY
CSB-EP007435CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Eukaryotic translation initiation factor 3 subunit I (Trip1) CSB-YP007538DLU
CSB-EP007538DLU
CSB-BP007538DLU
CSB-MP007538DLU
CSB-EP007538DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster UDP-glucose 6-dehydrogenase (sgl) CSB-YP025567DLU
CSB-EP025567DLU
CSB-BP025567DLU
CSB-MP025567DLU
CSB-EP025567DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L19 (rpl-19) CSB-YP020160CXY
CSB-EP020160CXY
CSB-BP020160CXY
CSB-MP020160CXY
CSB-EP020160CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix jacchus Lutropin-choriogonadotropic hormone receptor (LHCGR), partial CSB-YP012911CYL1
CSB-EP012911CYL1
CSB-BP012911CYL1
CSB-MP012911CYL1
CSB-EP012911CYL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae ATP synthase subunit a (MT-ATP6), partial CSB-YP015070LNS1
CSB-EP015070LNS1
CSB-BP015070LNS1
CSB-MP015070LNS1
CSB-EP015070LNS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae NADH-ubiquinone oxidoreductase chain 5 (MT-ND5), partial CSB-YP015081LNS
CSB-EP015081LNS
CSB-BP015081LNS
CSB-MP015081LNS
CSB-EP015081LNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071DRG1
CSB-EP015071DRG1
CSB-BP015071DRG1
CSB-MP015071DRG1
CSB-EP015071DRG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum Cytochrome b (MT-CYB), partial CSB-YP015075DRG
CSB-EP015075DRG
CSB-BP015075DRG
CSB-MP015075DRG
CSB-EP015075DRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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