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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Macaca arctoides C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844MOU1
CSB-EP004844MOU1
CSB-BP004844MOU1
CSB-MP004844MOU1
CSB-EP004844MOU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla dieffenbachii Cytochrome b (mt-cyb), partial CSB-YP015075BZG
CSB-EP015075BZG
CSB-BP015075BZG
CSB-MP015075BZG
CSB-EP015075BZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome b (MT-CYB), partial CSB-YP015075EWO
CSB-EP015075EWO
CSB-BP015075EWO
CSB-MP015075EWO
CSB-EP015075EWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taurotragus derbianus Cytochrome b (MT-CYB), partial CSB-YP015075TLL
CSB-EP015075TLL
CSB-BP015075TLL
CSB-MP015075TLL
CSB-EP015075TLL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhinolophus hipposideros Cytochrome b (MT-CYB), partial CSB-YP015075RKE1
CSB-EP015075RKE1
CSB-BP015075RKE1
CSB-MP015075RKE1
CSB-EP015075RKE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyrtonyx montezumae Cytochrome b (MT-CYB), partial CSB-YP015075EQF
CSB-EP015075EQF
CSB-BP015075EQF
CSB-MP015075EQF
CSB-EP015075EQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eulemur macaco flavifrons Cytochrome b (MT-CYB), partial CSB-YP015075EPF
CSB-EP015075EPF
CSB-BP015075EPF
CSB-MP015075EPF
CSB-EP015075EPF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus Cytochrome c oxidase subunit 1 (COI), partial CSB-YP015072RKI
CSB-EP015072RKI
CSB-BP015072RKI
CSB-MP015072RKI
CSB-EP015072RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus Cytochrome c oxidase subunit 3 (COIII), partial CSB-YP015074RKI1
CSB-EP015074RKI1
CSB-BP015074RKI1
CSB-MP015074RKI1
CSB-EP015074RKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076RKI
CSB-EP015076RKI
CSB-BP015076RKI
CSB-MP015076RKI
CSB-EP015076RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum tuberosum NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078FIG1
CSB-EP015078FIG1
CSB-BP015078FIG1
CSB-MP015078FIG1
CSB-EP015078FIG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 60S ribosomal protein L23 (rpl23a) CSB-YP020186SXV
CSB-EP020186SXV
CSB-BP020186SXV
CSB-MP020186SXV
CSB-EP020186SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans TATA-box-binding protein (TBP1) CSB-YP023239CZD
CSB-EP023239CZD
CSB-BP023239CZD
CSB-MP023239CZD
CSB-EP023239CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S ribosomal protein L18a (rpl-20) CSB-YP020155CXY
CSB-EP020155CXY
CSB-BP020155CXY
CSB-MP020155CXY
CSB-EP020155CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkyldihydroxyacetonephosphate synthase (ads-1), partial CSB-YP001457CXY
CSB-EP001457CXY
CSB-BP001457CXY
CSB-MP001457CXY
CSB-EP001457CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Protein MMS22-like (CG14803), partial CSB-YP004078DLU
CSB-EP004078DLU
CSB-BP004078DLU
CSB-MP004078DLU
CSB-EP004078DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60S acidic ribosomal protein P1 CSB-YP020340LOH
CSB-EP020340LOH
CSB-BP020340LOH
CSB-MP020340LOH
CSB-EP020340LOH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Liver carboxylesterase 1 (CES1), partial CSB-YP005258MOV
CSB-EP005258MOV
CSB-BP005258MOV
CSB-MP005258MOV
CSB-EP005258MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Acidic fibroblast growth factor intracellular-binding protein (FIBP) CSB-YP008671DSU
CSB-EP008671DSU
CSB-BP008671DSU
CSB-MP008671DSU
CSB-EP008671DSU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Connochaetes taurinus Cytochrome b (MT-CYB), partial CSB-YP015075DVR
CSB-EP015075DVR
CSB-BP015075DVR
CSB-MP015075DVR
CSB-EP015075DVR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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