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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Gazella dama Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074GEY1
CSB-EP015074GEY1
CSB-BP015074GEY1
CSB-MP015074GEY1
CSB-EP015074GEY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Centromere/kinetochore protein zw10 homolog (ZW10), partial CSB-YP027154DOA
CSB-EP027154DOA
CSB-BP027154DOA
CSB-MP027154DOA
CSB-EP027154DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Panax ginseng Squalene monooxygenase SE1 (SQE1), partial CSB-YP022645EQW1
CSB-EP022645EQW1
CSB-BP022645EQW1
CSB-MP022645EQW1
CSB-EP022645EQW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cicer arietinum Lactoylglutathione lyase CSB-YP009511DTI
CSB-EP009511DTI
CSB-BP009511DTI
CSB-MP009511DTI
CSB-EP009511DTI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays AP-2 complex subunit sigma (AP-17) CSB-YP001873ZAX
CSB-EP001873ZAX
CSB-BP001873ZAX
CSB-MP001873ZAX
CSB-EP001873ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus equinus ATP synthase subunit delta (atpH) CSB-YP002355FML
CSB-EP002355FML
CSB-BP002355FML
CSB-MP002355FML
CSB-EP002355FML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit beta (atpD) CSB-YP002350DNW
CSB-EP002350DNW
CSB-BP002350DNW
CSB-MP002350DNW
CSB-EP002350DNW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Adenosylhomocysteinase (ahcY) CSB-YP001474RLE
CSB-EP001474RLE
CSB-BP001474RLE
CSB-MP001474RLE
CSB-EP001474RLE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Ribosome-recycling factor (frr) CSB-YP014941BUD
CSB-EP014941BUD
CSB-BP014941BUD
CSB-MP014941BUD
CSB-EP014941BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum ATP synthase subunit delta (atpH) CSB-YP002355BXE
CSB-EP002355BXE
CSB-BP002355BXE
CSB-MP002355BXE
CSB-EP002355BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium acetobutylicum Triosephosphate isomerase (tpiA) CSB-YP024102DUB
CSB-EP024102DUB
CSB-BP024102DUB
CSB-MP024102DUB
CSB-EP024102DUB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium ammoniagenes GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DWW
CSB-EP009582DWW
CSB-BP009582DWW
CSB-MP009582DWW
CSB-EP009582DWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Serine palmitoyltransferase 1 (SPTLC1), partial CSB-YP022639DXU1
CSB-EP022639DXU1
CSB-BP022639DXU1
CSB-MP022639DXU1
CSB-EP022639DXU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus DNA repair protein XRCC1 (XRCC1), partial CSB-YP026229DXU
CSB-EP026229DXU
CSB-BP026229DXU
CSB-MP026229DXU
CSB-EP026229DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Apodemus flavicollis Sex-determining region Y protein (SRY) CSB-YP022699DNQ
CSB-EP022699DNQ
CSB-BP022699DNQ
CSB-MP022699DNQ
CSB-EP022699DNQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Cadherin-2 (CDH2) CSB-YP005045DXU
CSB-EP005045DXU
CSB-BP005045DXU
CSB-MP005045DXU
CSB-EP005045DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Glutaredoxin-2 (MVA073L, ACAM3000_MVA_073) CSB-YP009522VEE
CSB-EP009522VEE
CSB-BP009522VEE
CSB-MP009522VEE
CSB-EP009522VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Tolloid-like protein 2 (tll2), partial CSB-YP023596XBE
CSB-EP023596XBE
CSB-BP023596XBE
CSB-MP023596XBE
CSB-EP023596XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Gap junction alpha-1 protein (gja1), partial CSB-YP009443DIL1
CSB-EP009443DIL1
CSB-BP009443DIL1
CSB-MP009443DIL1
CSB-EP009443DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ambystoma mexicanum DNA nucleotidylexotransferase (DNTT), partial CSB-YP007088BYD
CSB-EP007088BYD
CSB-BP007088BYD
CSB-MP007088BYD
CSB-EP007088BYD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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