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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aquifex aeolicus 6-phosphofructokinase (pfkA) CSB-YP017822DNV
CSB-EP017822DNV
CSB-BP017822DNV
CSB-MP017822DNV
CSB-EP017822DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Translation initiation factor IF-3 (infC) CSB-YP015166DNV
CSB-EP015166DNV
CSB-BP015166DNV
CSB-MP015166DNV
CSB-EP015166DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA), partial CSB-YP017878DNV1
CSB-EP017878DNV1
CSB-BP017878DNV1
CSB-MP017878DNV1
CSB-EP017878DNV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase, partial CSB-YP009476TNR
CSB-EP009476TNR
CSB-BP009476TNR
CSB-MP009476TNR
CSB-EP009476TNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Sodium-dependent multivitamin transporter (Slc5a6), partial CSB-YP021683RA
CSB-EP021683RA
CSB-BP021683RA
CSB-MP021683RA
CSB-EP021683RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meriones unguiculatus Beta-3 adrenergic receptor (ADRB3), partial CSB-YP001393MRA1
CSB-EP001393MRA1
CSB-BP001393MRA1
CSB-MP001393MRA1
CSB-EP001393MRA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Homeobox protein MIXL1 CSB-YP014596CH
CSB-EP014596CH
CSB-BP014596CH
CSB-MP014596CH
CSB-EP014596CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paralichthys olivaceus Glucocorticoid receptor (nr3c1), partial CSB-YP016059ERT
CSB-EP016059ERT
CSB-BP016059ERT
CSB-MP016059ERT
CSB-EP016059ERT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serinus canaria Early growth response protein 1 (EGR1) CSB-YP007484SYL
CSB-EP007484SYL
CSB-BP007484SYL
CSB-MP007484SYL
CSB-EP007484SYL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Metalloproteinase inhibitor 3 (timp3) CSB-YP023562XBE
CSB-EP023562XBE
CSB-BP023562XBE
CSB-MP023562XBE
CSB-EP023562XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana pipiens Guanylyl cyclase-activating protein 2 (GUCA1B) CSB-YP010045RJN
CSB-EP010045RJN
CSB-BP010045RJN
CSB-MP010045RJN
CSB-EP010045RJN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Tyrosine-protein kinase receptor Tie-1 (tie1) CSB-YP023534DIL
CSB-EP023534DIL
CSB-BP023534DIL
CSB-MP023534DIL
CSB-EP023534DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis T-cell acute lymphocytic leukemia protein 1 (tal1) CSB-YP023110XBE
CSB-EP023110XBE
CSB-BP023110XBE
CSB-MP023110XBE
CSB-EP023110XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Blastobotrys adeninivorans Replication factor C subunit 3 (RFC3) CSB-YP019590BTJ
CSB-EP019590BTJ
CSB-BP019590BTJ
CSB-MP019590BTJ
CSB-EP019590BTJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Anaphase-promoting complex subunit 13 (apc13) CSB-YP001697SXV
CSB-EP001697SXV
CSB-BP001697SXV
CSB-MP001697SXV
CSB-EP001697SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Epsin-1 (ent1), partial CSB-YP007740SXV
CSB-EP007740SXV
CSB-BP007740SXV
CSB-MP007740SXV
CSB-EP007740SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe SET domain-containing protein 5 (set5) CSB-YP021106SXV
CSB-EP021106SXV
CSB-BP021106SXV
CSB-MP021106SXV
CSB-EP021106SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Phosphoglucomutase-2 (SPCC1840.05c), partial CSB-YP017867SXV
CSB-EP017867SXV
CSB-BP017867SXV
CSB-MP017867SXV
CSB-EP017867SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe IST1 homolog (SPCC1442.17c, SPCC285.02c) CSB-YP012171SXV
CSB-EP012171SXV
CSB-BP012171SXV
CSB-MP012171SXV
CSB-EP012171SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe U6 snRNA-associated Sm-like protein LSm7 (lsm7) CSB-YP013211SXV
CSB-EP013211SXV
CSB-BP013211SXV
CSB-MP013211SXV
CSB-EP013211SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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