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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe Vacuolar protein sorting-associated protein 35 (vps35), partial CSB-YP025904SXV
CSB-EP025904SXV
CSB-BP025904SXV
CSB-MP025904SXV
CSB-EP025904SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA mismatch repair protein msh2 (msh2), partial CSB-YP015028SXV
CSB-EP015028SXV
CSB-BP015028SXV
CSB-MP015028SXV
CSB-EP015028SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Origin recognition complex subunit 6 (orc6) CSB-YP017236SXV
CSB-EP017236SXV
CSB-BP017236SXV
CSB-MP017236SXV
CSB-EP017236SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Eukaryotic translation initiation factor 2A (SPBC4B4.04), partial CSB-YP007509SXV
CSB-EP007509SXV
CSB-BP007509SXV
CSB-MP007509SXV
CSB-EP007509SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (NDUFB1), partial CSB-YP015641HU
CSB-EP015641HU
CSB-BP015641HU
CSB-MP015641HU
CSB-EP015641HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Polycomb protein EED (EED) CSB-YP007408HU
CSB-EP007408HU
CSB-BP007408HU
CSB-MP007408HU
CSB-EP007408HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant S-adenosylmethionine decarboxylase proenzyme CSB-YP001658TQY
CSB-EP001658TQY
CSB-BP001658TQY
CSB-MP001658TQY
CSB-EP001658TQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster DNA repair and recombination protein RAD54-like (okr), partial CSB-YP019271DLU
CSB-EP019271DLU
CSB-BP019271DLU
CSB-MP019271DLU
CSB-EP019271DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Serine/threonine-protein phosphatase 4 catalytic subunit (Pp4-19C) CSB-YP018577DLU
CSB-EP018577DLU
CSB-BP018577DLU
CSB-MP018577DLU
CSB-EP018577DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Cryptochrome-1 (cry), partial CSB-YP006005DLU
CSB-EP006005DLU
CSB-BP006005DLU
CSB-MP006005DLU
CSB-EP006005DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lumbricus rubellus 40S ribosomal protein S13 (RPS13) CSB-YP020370LRJ
CSB-EP020370LRJ
CSB-BP020370LRJ
CSB-MP020370LRJ
CSB-EP020370LRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Galactoside 2-alpha-L-fucosyltransferase 2 (FUT2), partial CSB-YP009076GGZ1
CSB-EP009076GGZ1
CSB-BP009076GGZ1
CSB-MP009076GGZ1
CSB-EP009076GGZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rabbit T-complex protein 1 subunit zeta (CCT6), partial CSB-YP004865RB
CSB-EP004865RB
CSB-BP004865RB
CSB-MP004865RB
CSB-EP004865RB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dugong dugon Alpha-2B adrenergic receptor (ADRA2B), partial CSB-YP001389DMV1
CSB-EP001389DMV1
CSB-BP001389DMV1
CSB-MP001389DMV1
CSB-EP001389DMV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072DQG
CSB-EP015072DQG
CSB-BP015072DQG
CSB-MP015072DQG
CSB-EP015072DQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Carassius auratus NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078DQG1
CSB-EP015078DQG1
CSB-BP015078DQG1
CSB-MP015078DQG1
CSB-EP015078DQG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ateles paniscus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076DPI
CSB-EP015076DPI
CSB-BP015076DPI
CSB-MP015076DPI
CSB-EP015076DPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tamias sibiricus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076TLD
CSB-EP015076TLD
CSB-BP015076TLD
CSB-MP015076TLD
CSB-EP015076TLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macropus rufus NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076MPH
CSB-EP015076MPH
CSB-BP015076MPH
CSB-MP015076MPH
CSB-EP015076MPH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Perameles gunnii NADH-ubiquinone oxidoreductase chain 1 (MT-ND1), partial CSB-YP015076ETQ
CSB-EP015076ETQ
CSB-BP015076ETQ
CSB-MP015076ETQ
CSB-EP015076ETQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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